{"id":8132,"date":"2026-09-20T16:55:40","date_gmt":"2026-09-20T08:55:40","guid":{"rendered":"https:\/\/www.bio-oi.com\/?p=8132"},"modified":"2026-09-20T16:58:50","modified_gmt":"2026-09-20T08:58:50","slug":"%e5%b0%8f%e5%8a%a8%e7%89%a9%e6%b4%bb%e4%bd%93%e6%88%90%e5%83%8f%e5%ae%9e%e9%aa%8c%e4%b8%ad%e5%b8%b8%e8%a7%81%e9%97%ae%e9%a2%98%e5%8f%8a%e8%a7%a3%e5%86%b3%e6%96%b9%e6%a1%88","status":"publish","type":"post","link":"https:\/\/www.bio-oi.com\/?p=8132","title":{"rendered":"\u5c0f\u52a8\u7269\u6d3b\u4f53\u6210\u50cf\u5b9e\u9a8c\u4e2d\u5e38\u89c1\u95ee\u9898\u53ca\u89e3\u51b3\u65b9\u6848"},"content":{"rendered":"

\u81ea\u53d1\u5149\u6a21\u5f0f-\u5c0f\u9f20\u6210\u50cf\u5b8c\u5168\u6ca1\u4fe1\u53f7<\/span><\/strong><\/span><\/p>\n

\"\"<\/p>\n

 <\/p>\n

\u4e00\u3001\u5e95\u7269\u6ce8\u5c04\u4e0e\u5904\u7406<\/span><\/strong><\/span><\/p>\n

\u8fd9\u662f\u751f\u7269\u53d1\u5149\u5b9e\u9a8c\u6700\u5173\u952e\u7684\u4e00\u6b65\uff0c\u95ee\u9898\u4e5f\u6700\u5e38\u51fa\u5728\u8fd9\u91cc\u3002<\/span><\/strong><\/p>\n

\u2460\u5e95\u7269\u6d3b\u6027\uff1a<\/strong>D-\u8367\u5149\u7d20\u5e95\u7269\u5bf9\u5149\u548c\u6e29\u5ea6\u654f\u611f\u3002\u9700\u8981-20\u2103\u907f\u5149\u4fdd\u5b58\uff0c\u7528\u524d\u65b0\u9c9c\u914d\u5236\uff0c\u5e76\u4e14\u8981\u907f\u5149\u64cd\u4f5c\u3002\u53cd\u590d\u51bb\u878d\u6216\u914d\u5236\u540e\u653e\u7f6e\u592a\u4e45\u90fd\u4f1a\u5bfc\u81f4\u5e95\u7269\u964d\u89e3\uff0c\u4fe1\u53f7\u81ea\u7136\u5c31\u6ca1\u6709\u4e86\u3002<\/span><\/p>\n

\u2461\u5242\u91cf\u548c\u6d53\u5ea6\uff1a<\/strong>\u5e38\u89c4\u8179\u8154\u6ce8\u5c04\u5242\u91cf\u662f150mg\/kg\u4f53\u91cd\u3002\u53ef\u4ee5\u630910\u00b5l\/g\u7684\u6ce8\u5c04\u91cf\u6765\u63a8\u7b97\uff0c\u6bd4\u598220g\u7684\u5c0f\u9f20\u6ce8\u5c04200\u00b5l\u3002<\/span><\/p>\n

\u2462\u53cd\u5e94\u65f6\u95f4\uff1a<\/strong>\u8179\u8154\u6ce8\u5c04\u540e\uff0c\u9700\u8981\u7b49\u5f8510-20\u5206\u949f\uff0c\u8ba9\u5e95\u7269\u5728\u4f53\u5185\u5145\u5206\u53cd\u5e94\uff0c\u4fe1\u53f7\u624d\u80fd\u8fbe\u5230\u5cf0\u503c\u3002\u65f6\u95f4\u592a\u77ed\u6216\u592a\u957f\u4fe1\u53f7\u90fd\u4f1a\u5f88\u5f31\u3002\u5982\u679c\u4e0d\u786e\u5b9a\uff0c\u53ef\u4ee5\u505a\u9884\u5b9e\u9a8c\uff0c\u5728\u6ce8\u5c04\u540e5\u300110\u300115\u300120\u300130\u5206\u949f\u5206\u522b\u62cd\u4e00\u5f20\uff0c\u627e\u5230\u4fe1\u53f7\u6700\u5f3a\u7684\u65f6\u95f4\u70b9\u3002<\/span><\/p>\n

\u2463\u6ce8\u5c04\u65b9\u5f0f\uff1a<\/strong>\u8179\u8154\u6ce8\u5c04\uff08IP\uff09\u64cd\u4f5c\u7b80\u5355\uff0c\u4fe1\u53f7\u7a33\u5b9a\uff1b\u5c3e\u9759\u8109\u6ce8\u5c04\uff08IV\uff09\u4fe1\u53f7\u66f4\u5f3a\u3001\u66f4\u5feb\uff0c\u4f46\u64cd\u4f5c\u96be\u5ea6\u5927\u3002\u5982\u679c\u521a\u6362\u6ce8\u5c04\u65b9\u5f0f\uff0c\u53ef\u80fd\u9700\u8981\u91cd\u65b0\u6478\u7d22\u6700\u4f73\u6210\u50cf\u65f6\u95f4<\/span><\/p>\n

\"\"<\/p>\n

\u5e95\u7269\u6ce8\u5c04\u4e0e\u6210\u50cf\u65f6\u673a\u793a\u610f\u56fe<\/span><\/p>\n

 <\/p>\n

\u4e8c\u3001\u52a8\u7269\u51c6\u5907\u4e0e\u72b6\u6001<\/span><\/strong><\/p>\n

\u52a8\u7269\u672c\u8eab\u7684\u72b6\u6001\u4f1a\u6781\u5927\u5f71\u54cd\u6210\u50cf\u8d28\u91cf\u3002<\/span><\/p>\n

\u2460\u8131\u6bdb\u7a0b\u5ea6\uff1a<\/strong>\u6bdb\u53d1\u4f1a\u4e25\u91cd\u963b\u6321\u5149\u4fe1\u53f7\uff0c\u53ef\u80fd\u5bfc\u81f4\u8367\u5149\u5f3a\u5ea6\u964d\u4f4e50%\u4ee5\u4e0a\u3002\u6210\u50cf\u524d\u4e00\u5b9a\u8981\u5f7b\u5e95\u8131\u6bdb\uff0c\u5c24\u5176\u662f\u89c2\u5bdf\u90e8\u4f4d\u3002<\/span><\/p>\n

\u2461\u9972\u6599\u5e72\u6270\uff1a<\/strong>\u5e38\u89c4\u9f20\u7cae\u91cc\u7684\u82dc\u84ff\u7b49\u6210\u5206\u4f1a\u4ea7\u751f\u80cc\u666f\u8367\u5149\uff0c\u5e72\u6270\u6210\u50cf\u3002\u5982\u679c\u80cc\u666f\u5f88\u4eae\u6216\u8005\u4fe1\u53f7\u4e0d\u6e05\uff0c\u53ef\u4ee5\u8003\u8651\u7981\u98df\u6216\u6362\u6210\u65e0\u8367\u5149\u7684\u7279\u6b8a\u9972\u6599\u3002<\/span><\/p>\n

\u2462\u9ebb\u9189\u548c\u4fdd\u6e29\uff1a<\/strong>\u9ebb\u9189\u8fc7\u6df1\u4f1a\u6291\u5236\u5c0f\u9f20\u4ee3\u8c22\uff0c\u5f71\u54cd\u53d1\u5149\u53cd\u5e94\u3002\u540c\u65f6\uff0c\u6210\u50cf\u5e73\u53f0\u5fc5\u987b\u752837\u00b0C\u52a0\u70ed\u57ab\u4fdd\u6e29\uff0c\u4f53\u6e29\u8fc7\u4f4e\u540c\u6837\u4f1a\u5f71\u54cd\u4ee3\u8c22\u548c\u4fe1\u53f7\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u8131\u6bdb\u7a0b\u5ea6\u5bf9\u6210\u50cf\u7684\u5f71\u54cd<\/span><\/p>\n

\u56fe\u6ce8\uff1a\u53bb\u6bdb\u80fd\u663e\u8457\u63d0\u5347\u8868\u9762\u8367\u5149\u6210\u50cf\u4fe1\u53f7\uff1b\u6bdb\u53d1\u540c\u65f6\u963b\u6321\u6fc0\u53d1\u4e0e\u53d1\u5c04\u5149\uff0c\u8179\u90e8\u53bb\u6bdb\u540e\u6ce8\u5c04\u70b9\u8367\u5149\u660e\u663e\u589e\u5f3a\u3002<\/span><\/p>\n

 <\/p>\n

\u4e09\u3001\u4eea\u5668\u53c2\u6570\u8bbe\u7f6e<\/span><\/strong><\/span><\/p>\n

\u9519\u8bef\u7684\u8bbe\u7f6e\u4f1a\u8ba9\u5fae\u5f31\u7684\u4fe1\u53f7\u88ab\u5ffd\u7565\u3002<\/span><\/p>\n

\u2460\u66dd\u5149\u65f6\u957f\uff1a<\/strong>\u751f\u7269\u53d1\u5149\u4fe1\u53f7\u5f31\uff0c\u9700\u8981\u8f83\u957f\u7684\u66dd\u5149\u65f6\u95f4\uff0c\u901a\u5e38\u8bbe\u7f6e\u4e3a1-5\u5206\u949f\u3002\u5982\u679c\u81ea\u52a8\u66dd\u5149\uff08\u6700\u957f60\u79d2\uff09\u6ca1\u4fe1\u53f7\uff0c\u53ef\u4ee5\u5c1d\u8bd5\u624b\u52a8\u8bbe\u7f6e\u66f4\u957f\u7684\u66dd\u5149\u65f6\u95f4\uff0c\u6bd4\u5982120\u79d2\u3002<\/span><\/p>\n

\u2461\u6ee4\u5149\u7247\u548cBinning\u8bbe\u7f6e\uff1a<\/strong>\u751f\u7269\u53d1\u5149\u6210\u50cf\u5e94\u9009\u62e9Open\u6ee4\u5149\u7247\u3002\u4e3a\u4e86\u63d0\u9ad8\u7075\u654f\u5ea6\uff0c\u53ef\u4ee5\u8bbe\u7f6e\u8f83\u5927\u7684Binning\u503c\uff0c\u6bd4\u59824\u00d74\u62168\u00d78\u3002<\/span><\/p>\n

\u2462\u6ee4\u5149\u7247\uff1a<\/strong>\u5982\u679c\u662f\u8367\u5149\u6210\u50cf\uff0c\u9700\u8981\u786e\u4fdd\u6fc0\u53d1\/\u53d1\u5c04\u6ee4\u5149\u7247\u4e0e\u6240\u7528\u67d3\u6599\u7684\u6ce2\u957f\u5339\u914d\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u5149\u4eea\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf\u8f6f\u4ef6\u64cd\u4f5c\u754c\u9762\u622a\u56fe<\/span><\/p>\n

 <\/p>\n

\u56db\u3001\u6a21\u578b\u4e0e\u5bf9\u7167<\/span><\/strong><\/span><\/p>\n

\u5982\u679c\u4ee5\u4e0a\u6b65\u9aa4\u90fd\u786e\u8ba4\u65e0\u8bef\uff0c\u95ee\u9898\u53ef\u80fd\u51fa\u5728\u5b9e\u9a8c\u6a21\u578b\u672c\u8eab\u3002\u9633\u6027\/\u9634\u6027\u5bf9\u7167\u600e\u4e48\u505a\uff1f\u8fd9\u662f\u5224\u65ad\u95ee\u9898\u51fa\u5728\u54ea\u7684\u5173\u952e\u3002<\/span><\/p>\n

\u2460\u9633\u6027\u5bf9\u7167\uff1a<\/strong>\u7528\u5df2\u77e5\u80fd\u7a33\u5b9a\u53d1\u5149\u7684\u7ec6\u80de\u6216\u52a8\u7269\u6a21\u578b\uff0c\u5982\u679c\u9633\u6027\u5bf9\u7167\u4e5f\u6ca1\u4fe1\u53f7<\/strong>\uff0c\u8bf4\u660e\u5b9e\u9a8c\u4f53\u7cfb\uff08\u5e95\u7269\u3001\u4eea\u5668\u8bbe\u7f6e\u7b49\uff09\u6709\u95ee\u9898<\/strong>\u3002<\/span><\/p>\n

\u2461\u9634\u6027\u5bf9\u7167\uff1a<\/strong>\u7528\u672a\u6807\u8bb0\u7684\u666e\u901a\u5c0f\u9f20\uff0c\u7528\u6765\u8bc4\u4f30\u80cc\u666f\u4fe1\u53f7\u6c34\u5e73\u3002<\/span><\/p>\n

\u2462\u7ec6\u80de\u6807\u8bb0\u72b6\u6001\uff1a<\/strong>\u5982\u679c\u521a\u5efa\u7acb\u4e86\u65b0\u7684\u7a33\u8f6c\u7ec6\u80de\u7cfb<\/strong>\uff0c\u9700\u8981\u786e\u8ba4\u8367\u5149\u7d20\u9176\u57fa\u56e0\u662f\u5426\u6210\u529f\u6574\u5408\u5e76\u8868\u8fbe\u3002\u53ef\u4ee5\u5148\u505a\u4f53\u5916\uff08in vitro\uff09\u7684\u8367\u5149\u7d20\u9176\u6d3b\u6027\u68c0\u6d4b<\/strong>\uff08\u5982Western Blot\u6216\u8367\u5149\u7d20\u9176\u62a5\u544a\u57fa\u56e0\u68c0\u6d4b\uff09\uff0c\u786e\u8ba4\u7ec6\u80de\u672c\u8eab\u6ca1\u95ee\u9898\u518d\u7528\u4e8e\u6d3b\u4f53\u6210\u50cf\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u751f\u7269\u53d1\u5149\u6a21\u578b\u9633\u6027\u4e0e\u7a7a\u767d\u5bf9\u7167<\/span><\/p>\n

\u56fe\u6ce8\uff1a\u56fe\u793a\u4e3a\u751f\u7269\u53d1\u5149\u6210\u50cf\u5bf9\u7167\u6210\u50cf\u3002\u5de6\u4fa7luc\u8377\u7624\u5c0f\u9f20\u53ef\u68c0\u6d4b\u5230\u7279\u5f02\u6027\u751f\u7269\u53d1\u5149\u4fe1\u53f7\uff1b\u53f3\u4fa7Con\u7a7a\u767d\u5bf9\u7167\u5c0f\u9f20\u65e0\u660e\u663e\u53d1\u5149\u4fe1\u53f7\uff0c\u7528\u4e8e\u533a\u5206\u771f\u5b9e\u76ee\u6807\u4fe1\u53f7\u4e0e\u80cc\u666f\u566a\u58f0\uff0c\u9a8c\u8bc1\u6a21\u578b\u6709\u6548\u6027\u3002<\/span><\/p>\n

 <\/p>\n

\u8367\u5149\u6a21\u5f0f<\/span><\/strong><\/span><\/p>\n

\"\"<\/p>\n

 <\/p>\n

\u4e00\u3001\u76ee\u6807\u5f88\u5f31\uff0c\u51e0\u4e4e\u770b\u4e0d\u5230<\/span><\/strong><\/span><\/p>\n

\u4e3b\u8981\u539f\u56e0\uff1a<\/strong>
\n1. \u6ee4\u7247\u9009\u9519\uff1a\u6fc0\u53d1 \/ \u53d1\u5c04\u6ce2\u6bb5\u4e0e\u67d3\u6599\u4e0d\u5339\u914d\uff1b
\n2. \u63a2\u9488\u6d53\u5ea6\u504f\u4f4e\u3001\u63a2\u9488\u6dec\u706d\u3001\u964d\u89e3\uff1b
\n3. \u6bdb\u53d1\u906e\u6321\u3001\u672a\u8131\u6bdb\uff0c\u5149\u88ab\u5438\u6536\u6563\u5c04\uff1b
\n4. \u6df1\u5c42\u80bf\u7624 \/ \u9776\u7ec4\u7ec7\uff0c\u7ec4\u7ec7\u5438\u6536\u8870\u51cf\uff1b
\n5. \u76f8\u673a\u53c2\u6570\uff1a\u66dd\u5149\u65f6\u95f4\u592a\u77ed\u3001\u5149\u5708\u5c0f\u3001binning \u592a\u5c0f\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u6210\u50cf\u56fe\u9776\u533a\u8367\u5149\u4fe1\u53f7\u504f\u5f31
\n\u56fe\u6ce8\uff1a\u6d3b\u4f53\u8367\u5149\u6210\u50cf\u793a\u4f8b\uff0c\u9776\u533a\u4ec5\u6709\u5fae\u5f31\u8367\u5149\u4fe1\u53f7\uff0c\u63d0\u793a\u76ee\u6807\u4fe1\u53f7\u68c0\u51fa\u56f0\u96be\u3002<\/span><\/p>\n

\u89e3\u51b3\u65b9\u6848\uff1a<\/strong>
\n1. \u6838\u5bf9\u67d3\u6599\u5149\u8c31\uff0c\u786e\u8ba4\u6fc0\u53d1\u3001\u53d1\u5c04\u6ee4\u7247\uff1b
\n2. \u67d3\u6599\u907f\u5149\u4fdd\u5b58\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\uff1b\u4f18\u5316\u7ed9\u836f\u5242\u91cf\uff1b
\n3. \u6210\u50cf\u533a\u57df\u5f7b\u5e95\u5243\u6bdb + \u8131\u6bdb\u818f\u5904\u7406\uff1b
\n4. \u6df1\u5c42\u6837\u672c\u4f18\u5148\u9009\u7528\u8fd1\u7ea2\u5916\u67d3\u6599 Cy5.5\/Cy7<\/strong>\uff0c\u907f\u5f00\u7eff\u5149\u901a\u9053\uff1b
\n5. \u9002\u5ea6\u589e\u5927\u5149\u5708\u3001\u589e\u52a0\u66dd\u5149\uff0c\u5408\u7406\u8c03 binning\uff0c\u4e0d\u8981\u65e0\u9650\u62c9\u9ad8\u66dd\u5149\uff08\u653e\u5927\u566a\u58f0\uff09\u3002<\/span><\/p>\n

 <\/p>\n

\u4e8c\u3001\u80cc\u666f\u5f88\u9ad8\u3001\u4fe1\u566a\u6bd4\u5dee\uff0c\u6574\u5f20\u56fe\u5f88\u4eae\uff0c\u5206\u4e0d\u6e05\u76ee\u6807\u4fe1\u53f7<\/span><\/strong><\/span><\/p>\n

\u4e3b\u8981\u539f\u56e0\uff1a<\/strong>
\n1. \u52a8\u7269\u81ea\u53d1\u8367\u5149\uff08\u6bdb\u53d1\u3001\u76ae\u80a4\u80f6\u539f\u3001NADH\uff1b\u5c3f\u6db2\u7caa\u4fbf\u6c61\u67d3\u4f53\u8868\uff1b\u666e\u901a\u82dc\u84ff\u9972\u6599\u6d88\u5316\u9053\u8367\u5149\uff09\uff1b
\n2. \u63a2\u9488\u975e\u7279\u5f02\u6027\u5bcc\u96c6\u809d\u810f\u3001\u813e\u810f\uff1b
\n3. \u6fc0\u53d1\u5149\u6563\u5c04\u6f0f\u5149\uff1b
\n4. \u77ed\u6ce2\u957f\uff08GFP\/FITC\uff09\u672c\u8eab\u80cc\u666f\u5c31\u5927\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u81ea\u53d1\u8367\u5149\u9020\u6210\u9ad8\u80cc\u666f\uff0c\u5e72\u6270\u76ee\u6807\u8bc6\u522b
\n\u56fe\u6ce8\uff1a\u5c0f\u9f20\u6d3b\u4f53\u8367\u5149\u6210\u50cf\u5b9e\u4f8b\uff0c\u7a7a\u767d\u5c0f\u9f20\uff08\u5de6\uff09\u4f53\u8868\u5b58\u5728\u5927\u9762\u79ef\u81ea\u53d1\u8367\u5149\uff0c\u5c5e\u4e8e\u81ea\u53d1\u5149\u5e72\u6270\u4fe1\u53f7\uff0c\u6613\u8bef\u5224\u4e3a\u76ee\u6807\u4fe1\u53f7\u3002<\/span><\/p>\n

\u89e3\u51b3\u65b9\u6848\uff1a<\/strong>
\n1. \u6210\u50cf\u533a\u57df\u5145\u5206\u8131\u6bdb\uff1b\u4f53\u8868\u64e6\u5e72\u51c0\u5c3f\u6db2\u7caa\u4fbf\uff1b
\n2. \u8179\u90e8\u6210\u50cf\uff1a\u63d0\u524d 3-7 \u5929\u66f4\u6362\u65e0\u82dc\u84ff\u4f4e\u8367\u5149\u9972\u6599\uff1b
\n3. \u4f18\u5148\u5207\u6362\u8fd1\u7ea2\u5916\u7a97\u53e3\uff1e650 nm<\/strong>\uff0c\u5927\u5e45\u538b\u4f4e\u81ea\u53d1\u8367\u5149\uff1b
\n4. \u8bbe\u7f6e\u9634\u6027\u5bf9\u7167\uff08\u4e0d\u7ed9\u63a2\u9488\u7684\u540c\u54c1\u7cfb\u5c0f\u9f20\uff09\u505a\u80cc\u666f\u6263\u9664\uff1b
\n5. \u4f18\u5316\u6ee4\u7247\uff0c\u589e\u5927\u65af\u6258\u514b\u65af\u4f4d\u79fb\uff0c\u51cf\u5c11\u6fc0\u53d1\u5149\u6cc4\u9732\u3002<\/span><\/p>\n

 <\/p>\n

\u4e09\u3001\u51fa\u73b0\u5047\u9633\u6027\uff1a\u6ca1\u6709\u7ed9\u836f\u7684\u5c0f\u9f20\u4e5f\u51fa\u73b0\u660e\u663e\u8367\u5149\u4fe1\u53f7<\/span><\/strong><\/span><\/p>\n

\u4e3b\u8981\u539f\u56e0\uff1a<\/strong>
\n1. \u76ae\u6bdb\u3001\u6392\u6cc4\u7269\u3001\u57ab\u6599\u7684\u81ea\u53d1\u8367\u5149\uff1b
\n2. \u6210\u50cf\u53f0\u6709\u67d3\u6599\u6b8b\u7559\u6c61\u67d3\uff1b
\n3. \u6ee4\u7247\u4e32\u5149\uff0c\u6fc0\u53d1\u5149\u76f4\u63a5\u6f0f\u5165\u63a2\u6d4b\uff1b
\n4. \u77ed\u6ce2\u957f\u901a\u9053\u6781\u6613\u51fa\u73b0\u8be5\u95ee\u9898\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u8367\u5149\u6210\u50cf\u5047\u9633\u6027\u4fe1\u53f7\u793a\u4f8b\uff08\u7ed9\u836f\u9f20\u4e0e\u7a7a\u767d\u5bf9\u7167\u9f20\u5bf9\u6bd4\uff09<\/span><\/p>\n

\u89e3\u51b3\u65b9\u6848\uff1a<\/strong>
\n1. \u6bcf\u6b21\u5b9e\u9a8c\u7ed3\u675f\uff0c75% \u4e59\u9187\u64e6\u62ed\u6210\u50cf\u53f0\uff1b
\n2. \u5fc5\u987b\u8bbe\u7f6e\u7a7a\u767d\u52a8\u7269\u5bf9\u7167\u7ec4<\/strong>\uff1b
\n3. \u6821\u9a8c\u6ee4\u7247\u7ec4\uff1b\u4f18\u5148\u8fd1\u7ea2\u5916\u67d3\u6599\uff1b
\n4. \u4e0d\u8981\u628a\u5c3f\u6db2\u7caa\u4fbf\u7559\u5728\u6210\u50cf\u89c6\u91ce\u5185\u3002<\/span><\/p>\n

 <\/p>\n

\u56db\u3001\u591a\u901a\u9053\u6210\u50cf\uff1a\u901a\u9053\u4e4b\u95f4\u53d1\u751f\u4e32\u6270\uff08\u5149\u8c31\u4e32\u8272\uff09<\/span><\/strong><\/span><\/p>\n

\u4e3b\u8981\u539f\u56e0\uff1a<\/strong>\u4e24\u79cd\u67d3\u6599\u53d1\u5c04\u5149\u8c31\u4e92\u76f8\u91cd\u53e0\uff1b\u6ee4\u7247\u5e26\u5bbd\u9009\u62e9\u4e0d\u5408\u7406\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u4e24\u79cd\u8367\u5149\u67d3\u6599\u53d1\u5c04\u5149\u8c31\u91cd\u53e0\u5f15\u53d1\u901a\u9053\u5149\u8c31\u4e32\u6270\u793a\u610f\u56fe<\/span><\/p>\n

\u89e3\u51b3\u65b9\u6848\uff1a<\/strong>
\n1. \u4e24\u5957\u67d3\u6599\u53d1\u5c04\u6ce2\u957f\u5c3d\u91cf\u95f4\u9694\u2265100 nm\uff1b
\n2. \u91c7\u7528\u987a\u5e8f\u91c7\u96c6\u6a21\u5f0f\uff0c\u4e0d\u8981\u540c\u65f6\u53cc\u6fc0\u53d1\uff1b
\n3. \u505a\u5149\u8c31\u62c6\u5206\uff08\u5982\u6709\u5149\u8c31\u6a21\u5757\uff09\uff1b
\n4. \u5355\u67d3\u6599\u5bf9\u7167\uff0c\u505a\u4e32\u8272\u6821\u6b63\u77e9\u9635\u3002<\/span><\/p>\n

 <\/p>\n

\u4e94\u3001\u4fe1\u53f7\u4f4d\u7f6e\u5f02\u5e38\uff0c\u5206\u5e03\u548c\u9884\u671f\u4e0d\u4e00\u81f4<\/span><\/strong><\/span><\/p>\n

\u4e3b\u8981\u539f\u56e0\uff1a<\/strong>
\n1. \u6ce8\u5c04\u5931\u8d25\uff1a\u5c3e\u9759\u8109\u6e17\u6f0f\u3001\u8179\u8154\u6ce8\u5c04\u6f0f\u5230\u76ae\u4e0b\uff1b
\n2. \u63a2\u9488\u4f53\u5185\u4ee3\u8c22\uff0c\u7f51\u72b6\u5185\u76ae\u7cfb\u7edf\uff08\u809d\u813e\uff09\u5bcc\u96c6\uff1b
\n3. \u52a8\u7269\u4f53\u4f4d\u79fb\u52a8\uff1b
\n4. \u67d3\u6599\u805a\u96c6\u6c89\u6dc0\uff0c\u6ca1\u6709\u5145\u5206\u6eb6\u89e3<\/strong>\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u79bb\u4f53\u5668\u5b98\u8367\u5149\u6210\u50cf\u663e\u793a\u63a2\u9488\u975e\u7279\u5f02\u6027\u5bcc\u96c6
\n\u56fe\u6ce8\uff1a\u56fe\u793a\u4e3a\u5c0f\u9f20\u79bb\u4f53\u5668\u5b98\u8367\u5149\u6210\u50cf\uff0c\u9884\u671f\u9776\u4fe1\u53f7\u4f4d\u4e8e\u809d\u810f\uff0c\u4f46\u63a2\u9488\u5927\u91cf\u975e\u7279\u5f02\u6027\u5bcc\u96c6\u4e8e\u80a0\u9053\uff0c\u4fe1\u53f7\u5206\u5e03\u4e0e\u5b9e\u9a8c\u9884\u671f\u4e0d\u4e00\u81f4\uff0c\u9020\u6210\u76ee\u6807\u5224\u8bfb\u56f0\u96be\u3002<\/span><\/p>\n

\u89e3\u51b3\u65b9\u6848\uff1a<\/strong>
\n1. \u6ce8\u5c04\u5b8c\u6210\u786e\u8ba4\u65e0\u5916\u6e17\uff1b\u5c3e\u9759\u8109\u6ce8\u5c04\u786e\u8ba4\u56de\u62bd\u56de\u8840\uff1b
\n2. \u6478\u6e05\u63a2\u9488\u52a8\u529b\u5b66\uff0c\u9009\u6700\u4f73\u6210\u50cf\u65f6\u95f4\u70b9\uff1b
\n3. \u9ebb\u9189\u6df1\u5ea6\u5145\u8db3\uff0c\u56fa\u5b9a\u52a8\u7269\u4f53\u4f4d\uff1b
\n4. \u67d3\u6599\u5145\u5206\u6eb6\u89e3\uff0c\u907f\u514d\u6790\u51fa\u805a\u96c6\u3002<\/span><\/p>\n

 <\/p>\n

\u516d\u3001\u540c\u4e00\u6279\u52a8\u7269\uff0c\u7ec4\u95f4\u91cd\u590d\u6027\u5f88\u5dee\uff0c\u6570\u636e\u6ce2\u52a8\u5927<\/span><\/strong><\/span><\/p>\n

\u4e3b\u8981\u539f\u56e0\uff1a<\/strong>
\n1. \u7ed9\u836f\u64cd\u4f5c\u4e0d\u4e00\u81f4\uff08\u6ce8\u5c04\u5242\u91cf\u3001\u6e17\u6f0f\uff09\uff1b
\n2. \u8131\u6bdb\u7a0b\u5ea6\u4e0d\u4e00\u81f4\uff1b
\n3. \u6210\u50cf\u65f6\u95f4\u70b9\u4e0d\u7edf\u4e00\uff08\u63a2\u9488\u4f53\u5185\u4ee3\u8c22\u52a8\u529b\u5b66\u7a97\u53e3\u7a84\uff09\uff1b
\n4. \u6bcf\u6b21\u6210\u50cf\u53c2\u6570\u4e0d\u4e00\u81f4\uff1b
\n5. \u52a8\u7269\u4e2a\u4f53\u4ee3\u8c22\u5dee\u5f02\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u540c\u6279\u6b21\u5c0f\u9f20\u6d3b\u4f53\u8367\u5149\u6210\u50cf
\n\u56fe\u6ce8\uff1a\u56fe\u793a\u4e3a\u540c\u6279\u6b21\u8377\u7624\u5c0f\u9f20\u6d3b\u4f53\u8367\u5149\u6210\u50cf\uff0c\u80bf\u7624\u533a\u57df\u8367\u5149\u4fe1\u53f7\u6ce2\u52a8\u5927\uff0c\u7ec4\u95f4\u91cd\u590d\u6027\u5dee\uff1b\u5c3e\u90e8\u4e3a\u6ce8\u5c04\u4f4d\u70b9\u6b8b\u7559\u4fe1\u53f7\u3002<\/span><\/p>\n

\u89e3\u51b3\u65b9\u6848\uff1a<\/strong>
\n1. \u7edf\u4e00\u6ce8\u5c04\u65b9\u5f0f\u3001\u5242\u91cf\uff1b\u675c\u7edd\u6e17\u6f0f\uff1b
\n2. \u6240\u6709\u5c0f\u9f20\u540c\u4e00\u90e8\u4f4d\u6807\u51c6\u5316\u8131\u6bdb\uff1b
\n3. \u7ed9\u836f\u540e\u4e25\u683c\u56fa\u5b9a\u7b49\u5f85\u65f6\u95f4\u518d\u6210\u50cf\uff1b
\n4. \u540c\u4e00\u6279\u5b9e\u9a8c\u66dd\u5149\u3001\u5149\u6e90\u529f\u7387\u3001binning \u5168\u90e8\u9501\u5b9a\uff0c\u4e0d\u968f\u610f\u6539\u52a8<\/strong>\uff1b
\n5. \u589e\u52a0\u751f\u7269\u5b66\u91cd\u590d\u3002<\/span><\/p>\n

 <\/p>\n

\u4e03\u3001\u53ea\u80fd\u770b\u8868\u5c42\uff0c\u6df1\u90e8\u75c5\u7076\u51e0\u4e4e\u68c0\u6d4b\u4e0d\u5230<\/span><\/strong><\/span><\/p>\n

\u4e3b\u8981\u539f\u56e0\uff1a<\/strong>\u53ef\u89c1\u5149\uff08\u7ea2\u3001\u7eff\u3001\u84dd\uff09\u88ab\u8840\u7ea2\u86cb\u767d\u5927\u91cf\u5438\u6536\u6563\u5c04\uff1b\u7ec4\u7ec7\u7a7f\u900f\u5dee\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u751f\u7269\u7ec4\u7ec7\u5bf9\u4e0d\u540c\u6ce2\u957f\u5149\u7684\u5438\u6536\u7279\u6027\u793a\u610f\u56fe<\/span><\/p>\n

\u89e3\u51b3\u65b9\u6848\uff1a<\/strong>
\n1. >650 nm \u8fd1\u7ea2\u5916\u7a97\u53e3\u67d3\u6599 Cy5.5\u3001Cy7\u3001ICG\uff1b
\n2. \u964d\u4f4e\u77ed\u6ce2\u957f\u8367\u5149\uff08GFP\/FITC\uff09\u7528\u4e8e\u6df1\u90e8\u4f53\u5185\u68c0\u6d4b\u7684\u9884\u671f\uff1b
\n3. \u6d45\u5c42\u76ae\u4e0b\u80bf\u7624\u53ef\u4ee5\u7528\u7eff\u5149\u901a\u9053\u3002<\/span><\/p>\n

 <\/p>\n

\u516b\u3001\u63a2\u9488\u5149\u7167\u6dec\u706d\uff0c\u591a\u6b21\u91c7\u96c6\u4fe1\u53f7\u8d8a\u6765\u8d8a\u4f4e<\/span><\/strong><\/span><\/p>\n

\u4e3b\u8981\u539f\u56e0\uff1a<\/strong>\u957f\u65f6\u95f4\u5f3a\u6fc0\u53d1\u5149\u7167\u5c04\uff0c\u5149\u6f02\u767d\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u8fde\u7eed\u591a\u6b21\u6fc0\u53d1\u91c7\u96c6\u4fe1\u53f7\u8870\u51cf<\/span><\/p>\n

\u89e3\u51b3\u65b9\u6848\uff1a<\/strong>\u51cf\u5c11\u6fc0\u53d1\u5149\u529f\u7387\uff0c\u7f29\u77ed\u5355\u6b21\u66dd\u5149\uff1b\u95f4\u9694\u91c7\u96c6\uff0c\u4e0d\u8981\u8fde\u7eed\u53cd\u590d\u6fc0\u53d1\u540c\u4e00\u4f4d\u7f6e\uff1b\u9009\u62e9\u6297\u5149\u6f02\u767d\u66f4\u597d\u7684\u63a2\u9488\u3002<\/span><\/p>\n

 <\/p>\n

\u76f8\u5173\u4ea7\u54c1<\/span><\/strong><\/span><\/p>\n

\"\"<\/a><\/p>\n

\u25b7 \u53cc\u6a21\u6210\u50cf\uff1a<\/strong><\/span>\u652f\u6301\u751f\u7269\u53d1\u5149\uff0b\u8367\u5149\uff0c\u4e00\u952e\u5207\u6362\u663e\u793a\u5355\u4f4d\uff08P\/S\u3001\u8f90\u4eae\u5ea6\u3001\u8367\u5149\u6548\u7387\u3001\u7070\u5ea6\u503c\uff09\u3002
\n\u25b6 \u8367\u5149\u6548\u7387\u76f4\u7b97\uff1a<\/strong><\/span>\u5185\u7f6e\u6fc0\u53d1\u7167\u5ea6\u6807\u5b9a\u4e0e\u4f4d\u7f6e\u6821\u6b63\uff0c\u8367\u5149\u5b9e\u9a8c\u53ef\u4ee5\u76f4\u63a5\u8f93\u51fa\u8367\u5149\u6548\u7387\uff0c\u8de8\u52a8\u7269\u3001\u8de8\u5929\u5bf9\u6bd4\u66f4\u7701\u5fc3\u3002
\n\u25b7 \u89c6\u91ce\u4e0e\u6e29\u63a7\uff1a<\/strong><\/span>\u7535\u52a8\u5347\u964d\u53f0\u9884\u8bbe3\u6863\u9ad8\u5ea6\uff0c\u5207\u6362\u89c6\u91ce\u5feb\uff1b\u8f7d\u6837\u53f0\u6052\u6e29\u52a0\u70ed\uff0c\u5e76\u517c\u5bb9\u5438\u5165\u9ebb\u9189\u63a5\u53e3\u3002
\n\u25b6 \u5b9a\u91cf\u53cb\u597d\u8f6f\u4ef6\uff1a<\/strong><\/span>ROI\u6846\u9009\u3001\u80cc\u666f\u81ea\u52a8\u6263\u9664\u5373\u53ef\u51fa\u7ed3\u679c\uff1b\u652f\u6301\u6279\u91cf\u5206\u6790\u3001\u53c2\u6570\u6a21\u677f\u590d\u7528\uff0c\u539f\u59cb\u6570\u636e\u548c\u62a5\u544a\u56fe\u53ef\u540c\u65f6\u5bfc\u51fa\u3002
\n\u25b7 \u5149\u5b66\u6269\u5c55\uff1a<\/strong><\/span>\u57fa\u7840 400-800nm \u6807\u914d\uff0c\u591a\u901a\u9053\u6ee4\u7247\u53ef\u6269\u5c55\u5230\u8fd1\u7ea2\u5916\uff08900-1700nm\uff09\uff0c\u8fd8\u53ef\u52a0\u88c5X\u5149\u6a21\u5757\uff0c\u6ee1\u8db3\u5149\u8c31\u5206\u79bb\u548c\u591a\u8272\u6210\u50cf\u9700\u6c42\u3002
\n\u25b6 \u81ea\u52a8\u8bb0\u5f55\uff1a<\/strong><\/span>\u66dd\u5149\u3001\u589e\u76ca\u3001\u6ee4\u7247\u548c\u51e0\u4f55\u4fe1\u606f\u90fd\u4f1a\u81ea\u52a8\u4fdd\u5b58\uff0c\u4fdd\u8bc1\u5b9e\u9a8c\u53e3\u5f84\u4e00\u81f4\uff0c\u590d\u73b0\u5b9e\u9a8c\u66f4\u5b89\u5fc3\u3002<\/span><\/p>\n

 <\/p>\n

 <\/p>\n

\u90e8\u5206\u53c2\u8003\u6587\u732e\uff1a<\/span><\/strong><\/span><\/p>\n

[1] Su, Y., Walker, J. R., Park, Y., Smith, T. P., Liu, L. X., Hall, M. P., Labanieh, L., Hurst, R., Wang, D. C., Encell, L. P., Kim, N., Zhang, F., Kay, M. A., Casey, K. M., Majzner, R. G., Cochran, J. R., Mackall, C. L., Kirkland, T. A., & Lin, M. Z. (2020). Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals. Nature Methods, 17(8), 852\u2013860. https:\/\/doi.org\/10.1038\/s41592-020-0889-6<\/span><\/p>\n

[2] Fichtner, J., Wagenh\u00e4user, Y., S\u00e1rosi, M., Stolte, M., & W\u00fcrthner, F. (2026). Fluorescent merocyanines: from fundamental properties to applications as molecular probes, in bioimaging and as emissive dye aggregates. Chemical Society Reviews. https:\/\/doi.org\/10.1039\/D6CS00194G<\/span><\/p>\n

[3] Mu, H., Miki, K., & Ohe, K. (2021). pH-Activatable cyanine dyes for selective tumor imaging using near-infrared fluorescence and photoacoustic modalities. ACS Sensors, 6(1), 123\u2013129. https:\/\/doi.org\/10.1021\/acssensors.0c01926<\/span><\/p>\n

 <\/p>\n

\u90e8\u5206\u56fe\u7247\u6765\u6e90\uff1a<\/span><\/strong><\/span><\/p>\n

[1] https:\/\/resources.revvity.com\/pdfs\/tch-ivis-spectrumct-general-and-technical-considerations-for-2D-and-3D-imaging.pdf\uff08Figure 6\uff09<\/span><\/p>\n

[2] Li L, et al. Engineered \u03b1v\u03b26-targeting peptide\u2012BSH conjugates for boron neutron capture therapy of pancreatic ductal adenocarcinoma [J]. Acta Pharmaceutica Sinica B, 2026.\uff08Figure 5\uff09<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u81ea\u53d1\u5149\u6a21\u5f0f-\u5c0f\u9f20\u6210\u50cf\u5b8c\u5168\u6ca1\u4fe1\u53f7   \u4e00\u3001\u5e95\u7269\u6ce8\u5c04\u4e0e\u5904\u7406 \u8fd9\u662f\u751f\u7269\u53d1\u5149\u5b9e\u9a8c\u6700\u5173\u952e\u7684\u4e00\u6b65\uff0c\u95ee\u9898\u4e5f\u6700\u5e38\u51fa\u5728 […]<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"footnotes":""},"categories":[94,90],"tags":[],"class_list":["post-8132","post","type-post","status-publish","format-standard","hentry","category-qw3","category-newcenter"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.bio-oi.com\/index.php?rest_route=\/wp\/v2\/posts\/8132","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bio-oi.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bio-oi.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bio-oi.com\/index.php?rest_route=\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bio-oi.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8132"}],"version-history":[{"count":8,"href":"https:\/\/www.bio-oi.com\/index.php?rest_route=\/wp\/v2\/posts\/8132\/revisions"}],"predecessor-version":[{"id":8157,"href":"https:\/\/www.bio-oi.com\/index.php?rest_route=\/wp\/v2\/posts\/8132\/revisions\/8157"}],"wp:attachment":[{"href":"https:\/\/www.bio-oi.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8132"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bio-oi.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8132"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bio-oi.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8132"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}