{"id":8099,"date":"2026-07-31T14:09:48","date_gmt":"2026-07-31T06:09:48","guid":{"rendered":"http:\/\/www.bio-oi.com\/?p=8099"},"modified":"2026-08-13T13:40:04","modified_gmt":"2026-08-13T05:40:04","slug":"%e6%b4%bb%e4%bd%93%e6%88%90%e5%83%8f%e5%ae%9e%e9%aa%8c%e6%96%b9%e6%b3%95%e5%88%86%e7%b1%bb%e6%80%bb%e7%bb%93%e5%8f%8a%e6%a0%b8%e5%bf%83%e5%ba%94%e7%94%a8","status":"publish","type":"post","link":"https:\/\/www.bio-oi.com\/en\/2026\/07\/31\/%e6%b4%bb%e4%bd%93%e6%88%90%e5%83%8f%e5%ae%9e%e9%aa%8c%e6%96%b9%e6%b3%95%e5%88%86%e7%b1%bb%e6%80%bb%e7%bb%93%e5%8f%8a%e6%a0%b8%e5%bf%83%e5%ba%94%e7%94%a8\/","title":{"rendered":"\u6d3b\u4f53\u6210\u50cf\u5b9e\u9a8c\u65b9\u6cd5\u5206\u7c7b\u603b\u7ed3\u53ca\u6838\u5fc3\u5e94\u7528"},"content":{"rendered":"
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\u4ec0\u4e48\u662f\u6d3b\u4f53\u6210\u50cf<\/span><\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u6d3b\u4f53\u6210\u50cf\u6307\u5728\u6d3b\u4f53\u72b6\u6001\u4e0b\u5728\u7ec6\u80de\u548c\u5206\u5b50\u6c34\u5e73\u4e0a\u5e94\u7528\u5f71\u50cf\u5b66\u65b9\u6cd5\u5bf9\u751f\u7269\u8fc7\u7a0b\u548c\u65f6\u95f4\u4e0a\u7684\u5b9a\u6027\u548c\u5b9a\u91cf\u5206\u6790\u7684\u4e00\u95e8\u79d1\u5b66\uff0c\u4e3b\u8981\u5305\u62ec\u751f\u7269\u53d1\u5149\u4e0e\u8367\u5149\u3001\u540c\u4f4d\u7d20\u6210\u50cf\u3001X\u5149\u6210\u50cf\u7b49\u3002\u5176\u4e2d\uff0c\u751f\u7269\u53d1\u5149\u662f\u8367\u5149\u7d20\u9176\u57fa\u56e0\u6807\u8bb0\u7ec6\u80de\uff0c\u800c\u8367\u5149\u6280\u672f\u5219\u91c7\u7528\u8367\u5149\u62a5\u544a\u57fa\u56e2\u8868\u8fbe\u7684\u8367\u5149\u86cb\u767d\u3001\u8367\u5149\u67d3\u6599\u7b49\u8fdb\u884c\u6807\u8bb0\uff0c\u7136\u540e\u5229\u7528\u4eea\u5668\u8fdb\u884c\u68c0\u6d4b\u3002\u901a\u8fc7\u6d3b\u4f53\u6210\u50cf\u6280\u672f\u53ef\u4ee5\u89c2\u6d4b\u6d3b\u4f53\u52a8\u7269\u4f53\u5185\u80bf\u7624\u7684\u751f\u957f\u53ca\u8f6c\u79fb\u3001\u611f\u67d3\u6027\u75be\u75c5\u53d1\u5c55\u8fc7\u7a0b\u3001\u7279\u5b9a\u57fa\u56e0\u7684\u8868\u8fbe\u7b49\u751f\u7269\u5b66\u8fc7\u7a0b\uff0c\u5176\u4e2d\u4ee5\u751f\u7269\u53d1\u5149\u5e94\u7528\u8f83\u4e3a\u5b9e\u7528\u3002<\/span><\/p>\n
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\u6309\u6210\u50cf\u539f\u7406\u5206\u7c7b<\/span><\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u5149\u5b66\u6210\u50cf\u662f\u76ee\u524d\u6700\u4e3b\u6d41\u3001\u5e94\u7528\u6700\u5e7f\u7684\u6280\u672f\uff0c\u5c24\u5176\u9002\u5408\u5728\u7ec6\u80de\u548c\u5206\u5b50\u6c34\u5e73\u8fdb\u884c\u7814\u7a76\u3002\u4e3b\u8981\u5206\u4e3a\u751f\u7269\u53d1\u5149\u548c\u8367\u5149\u6210\u50cf\u4e24\u7c7b\u4ee5\u53ca\u5176\u4ed6\u4f53\u5185\u6210\u50cf\u3002<\/span><\/p>\n
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\u6309\u7814\u7a76\u5bf9\u8c61\u5206\u7c7b<\/span><\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u6d3b\u4f53\u6210\u50cf\u6280\u672f\u7684\u5e94\u7528\u8303\u56f4\u975e\u5e38\u5e7f\uff0c\u7814\u7a76\u5bf9\u8c61\u4e5f\u591a\u79cd\u591a\u6837\uff1a<\/span>1\u3001\u6a21\u578b\u52a8\u7269\uff1a\u5982\u5c0f\u9f20\u3001\u5927\u9f20\u3001\u6591\u9a6c\u9c7c\u7b49\uff0c\u662f\u75be\u75c5\u6a21\u578b\u548c\u836f\u7269\u7b5b\u9009\u4e2d\u6700\u5e38\u7528\u7684\u5bf9\u8c61\u3002<\/span>2\u3001\u690d\u7269\uff1a\u5982\u62df\u5357\u82a5\u3001\u70df\u8349\u7b49\uff0c\u7528\u4e8e\u690d\u7269\u7814\u7a76\u5728\u5e72\u65f1\u3001\u76d0\u80c1\u8feb\u7b49\u6761\u4ef6\u4e0b\u7684\u751f\u7406\u53cd\u5e94\u548c\u75c5\u539f\u4fb5\u67d3\u8fc7\u7a0b\u3002<\/span>3\u3001\u7ec6\u80de\u548c\u7ec4\u7ec7\uff1a\u867d\u7136\u4e0d\u5b8c\u5168\u662f”\u6d3b\u4f53”\uff0c\u4f46\u79bb\u4f53\u7684\u7ec4\u7ec7\u6d3b\u7ec6\u80de\u6837\u672c\u4e5f\u53ef\u4ee5\u7528\u7c7b\u4f3c\u6280\u672f\u8fdb\u884c\u6210\u50cf\u5206\u6790\u3002<\/span><\/p>\n

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\u6309\u68c0\u6d4b\u5185\u5bb9\u5206\u7c7b<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\n
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\u6839\u636e\u7814\u7a76\u76ee\u7684\uff0c\u6d3b\u4f53\u6210\u50cf\u53ef\u4ee5\u68c0\u6d4b\u4e0d\u540c\u7684\u751f\u7269\u5b66\u5b9e\u9a8c\uff1a<\/span><\/p>\n
1\u3001\u57fa\u56e0\u8868\u8fbe\u4e0e\u86cb\u767d\u6fc0\u6d3b\uff1a<\/strong><\/span>\u89c2\u5bdf\u7279\u5b9a\u57fa\u56e0\u5728\u4f55\u65f6\u4f55\u5730\u88ab\u6fc0\u6d3b\u3002<\/span>2\u3001\u7ec6\u80de\u884c\u4e3a\uff1a<\/strong><\/span>\u8ffd\u8e2a\u7ec6\u80de\u7684\u8fc1\u79fb\u3001\u589e\u6b96\u3001\u51cb\u4ea1\u548c\u5206\u5e03\u3002<\/span><\/p>\n

3\u3001\u75be\u75c5\u8fdb\u5c55\uff1a<\/strong><\/span>\u76d1\u6d4b\u80bf\u7624\u7684\u751f\u957f\u4e0e\u8f6c\u79fb\u3001\u708e\u75c7\u611f\u67d3\u7076\u7684\u6269\u6563\u7b49\u3002<\/span><\/p>\n

4\u3001\u836f\u7269\u54cd\u5e94\uff1a<\/strong><\/span>\u8bc4\u4f30\u836f\u7269\u6216\u6cbb\u7597\u65b9\u6848\u7684\u6548\u679c\uff0c\u901a\u8fc7\u7eb5\u5411\u89c2\u5bdf\u4e00\u53ea\u52a8\u7269\uff0c\u51cf\u5c11\u4e2a\u4f53\u5dee\u5f02\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u6838\u5fc3\u5e94\u7528\u65b9\u5411<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\n
\u65e0\u8bba\u91c7\u7528\u54ea\u79cd\u6280\u672f\uff0c\u6d3b\u4f53\u6210\u50cf\u7684\u6838\u5fc3\u4ef7\u503c\u5728\u4e8e\u80fd\u591f\u5728\u4e0d\u4f24\u5bb3\u52a8\u7269\u7684\u524d\u63d0\u4e0b\uff0c\u5bf9\u540c\u4e00\u4e2a\u4f53\u8fdb\u884c\u957f\u671f\u3001\u52a8\u6001\u7684\u89c2\u6d4b\uff0c\u4ece\u800c\u5c06\u770b\u4e0d\u89c1\u7684\u751f\u547d\u8fc7\u7a0b\u8f6c\u5316\u4e3a\u53ef\u89c6\u5316\u7684\u6570\u636e\u3002<\/span><\/section>\n
<\/section>\n
1\u3001\u80bf\u7624\u7814\u7a76\uff1a<\/strong><\/span>\u5b9e\u65f6\u76d1\u6d4b\u80bf\u7624\u7ec6\u80de\u7684\u589e\u6b96\u3001\u8f6c\u79fb\u548c\u836f\u7269\u54cd\u5e94\u3002<\/span><\/section>\n
2\u3001\u836f\u7269\u7814\u53d1\uff1a<\/strong><\/span>\u8ffd\u8e2a\u836f\u7269\u5728\u4f53\u5185\u7684\u5206\u5e03\u3001\u4ee3\u8c22\u8fc7\u7a0b\u53ca\u9776\u5411\u6548\u679c\u3002<\/span>3\u3001\u611f\u67d3\u4e0e\u514d\u75ab\uff1a<\/strong><\/span>\u89c2\u5bdf\u7ec6\u83cc\u6216\u75c5\u6bd2\u5728\u4f53\u5185\u7684\u6269\u6563\uff0c\u4ee5\u53ca\u514d\u75ab\u7ec6\u80de\u7684\u8fc1\u79fb\u548c\u5e94\u7b54\u3002<\/span><\/p>\n

4\u3001\u57fa\u56e0\u8868\u8fbe\u5206\u6790\uff1a<\/strong><\/span>\u7814\u7a76\u7279\u5b9a\u57fa\u56e0\u5728\u4f55\u65f6\u3001\u4f55\u5730\u88ab\u6fc0\u6d3b\u3002<\/span><\/p>\n<\/section>\n<\/section>\n

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\u5b9e\u9a8c\u600e\u4e48\u9009\uff1f<\/span><\/strong><\/p>\n

\"\"<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

1\u3001\u8ffd\u6c42\u8d85\u9ad8\u7075\u654f\u5ea6\u548c\u5b9a\u91cf\uff1a<\/strong><\/span>\u9009\u751f\u7269\u53d1\u5149\uff08BLI\uff09\uff0c\u80cc\u666f\u5e72\u51c0\uff0c\u4fe1\u53f7\u7a33\u5b9a\uff0c\u9002\u5408\u957f\u671f\u76d1\u6d4b\u3002<\/span>2\u3001\u9700\u8981\u591a\u9776\u70b9\u6216\u9ad8\u5206\u8fa8\u7387\uff1a<\/strong><\/span>\u9009\u8367\u5149\u6210\u50cf\uff08FLI\uff09\uff0c\u6807\u8bb0\u7075\u6d3b\uff0c\u53ef\u591a\u8272\u6210\u50cf\uff0c\u80fd\u770b\u6e05\u7ed3\u6784\u7ec6\u8282\u3002<\/span><\/p>\n

3\u3001\u8981\u770b\u7cbe\u7ec6\u89e3\u5256\u7ed3\u6784\uff1a<\/strong><\/span>\u9009 MRI\u6216CT\uff0c\u80fd\u6e05\u6670\u5448\u73b0\u5668\u5b98\u548c\u7ec4\u7ec7\u5f62\u6001\u3002<\/span><\/p>\n<\/section>\n

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\u751f\u7269\u53d1\u5149\u6210\u50cf\u5e95\u7269\u5982\u4f55\u9009\u62e9\uff1f<\/span><\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u4e3b\u6d41\u9009\u62e9\uff1aD-\u8367\u5149\u7d20<\/span><\/strong><\/section>\n

D-\u8367\u5149\u7d20\uff08D-Luciferin\uff09<\/strong>\u662f\u8424\u706b\u866b\u8367\u5149\u7d20\u9176\uff08Firefly Luciferase\uff09\u7cfb\u7edf\u7684\u4e13\u7528\u5e95\u7269\uff0c\u4e5f\u662f\u76ee\u524d\u6d3b\u4f53\u6210\u50cf\u4e2d\u6700\u4e3b\u6d41\u7684\u9009\u62e9\u3002<\/span><\/p>\n

\u4f18\u70b9\uff1a<\/span>\u5b83\u53d1\u51fa\u7684\u5149\u6ce2\u957f\u5728540-600nm\uff0c\u5c5e\u4e8e\u504f\u7ea2\u5149\uff0c\u5728\u751f\u7269\u7ec4\u7ec7\u4e2d\u7684\u7a7f\u900f\u6027\u66f4\u597d\uff0c\u4fe1\u566a\u6bd4\u9ad8\u3002\u4fe1\u53f7\u7a33\u5b9a\uff0c\u7279\u5f02\u6027\u597d\uff0c\u975e\u5e38\u9002\u5408\u7528\u4e8e\u80bf\u7624\u751f\u957f\u3001\u836f\u7269\u7597\u6548\u8bc4\u4f30\u7b49\u9700\u8981\u957f\u671f\u52a8\u6001\u76d1\u6d4b\u7684\u5b9e\u9a8c\u3002<\/span><\/p>\n

\u9002\u7528\u573a\u666f\uff1a<\/span>\u7edd\u5927\u591a\u6570\u57fa\u4e8eLuc\u62a5\u544a\u57fa\u56e0\u7684\u6d3b\u4f53\u6210\u50cf\u5b9e\u9a8c\uff0c\u5982\u80bf\u7624\u6a21\u578b\u3001\u5e72\u7ec6\u80de\u793a\u8e2a\u3001\u611f\u67d3\u6027\u75be\u75c5\u7814\u7a76\u7b49\u3002<\/span><\/p>\n<\/section>\n<\/section>\n

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\u00a0\u5176\u4ed6\u5e38\u89c1\u5e95\u7269<\/span><\/strong><\/span><\/section>\n

\u9664\u4e86D-\u8367\u5149\u7d20\uff0c\u6839\u636e\u4f60\u4f7f\u7528\u7684\u8367\u5149\u7d20\u9176\u4e0d\u540c\uff0c\u8fd8\u6709\u5176\u4ed6\u9009\u62e9\u3002<\/span><\/p>\n

\u8154\u80a0\u7d20\uff08Coelenterazine\uff09\uff1a<\/span><\/strong>\u8fd9\u662f\u6d77\u80be\u8367\u5149\u7d20\u9176\uff08Renilla Luciferase\uff09\u7684\u5e95\u7269\u3002\u5b83\u7684\u53d1\u5149\u6ce2\u957f\u8f83\u77ed\uff08460-540nm\uff09\uff0c\u5728\u4f53\u5185\u4ee3\u8c22\u5feb\uff0c\u4fe1\u53f7\u6301\u7eed\u65f6\u95f4\u77ed\uff0c\u901a\u5e38\u7528\u4e8e\u53cc\u62a5\u544a\u57fa\u56e0\u5b9e\u9a8c\u6216 BRET \u7814\u7a76\u3002<\/span><\/p>\n

Furimazine\uff1a<\/span><\/strong>\u8fd9\u662f\u4e00\u79cd\u65b0\u578b\u5e95\u7269\uff0c\u4e0e NanoLuc \u8367\u5149\u7d20\u9176\u8054\u7528\u3002\u5b83\u7684\u4eae\u5ea6\u6781\u9ad8\uff0c\u4fe1\u53f7\u6301\u7eed\u65f6\u95f4\u957f\uff0c\u7075\u654f\u5ea6\u8fdc\u8d85\u4f20\u7edf\u5e95\u7269\uff0c\u975e\u5e38\u9002\u5408\u7528\u4e8e\u68c0\u6d4b\u4f4e\u4e30\u5ea6\u9776\u70b9\u6216\u9700\u8981\u9ad8\u7075\u654f\u5ea6\u7684\u573a\u666f\u3002<\/span><\/p>\n<\/section>\n<\/section>\n

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\u5b9e\u9a8c\u907f\u5751\u5c0f\u8d34\u58eb<\/span><\/strong><\/span><\/p>\n<\/section>\n<\/section>\n

1. \u6eb6\u89e3\u7f13\u51b2\u6db2\uff1a<\/strong><\/span>\u914d\u5236D-\u8367\u5149\u7d20\u6eb6\u6db2\u65f6\uff0c\u6700\u597d\u7528\u4e0d\u542b\u9499\u9541\u79bb\u5b50\u7684 PBS\uff08\u5982 DPBS\uff09\uff0c\u56e0\u4e3a\u8fd9\u4e9b\u79bb\u5b50\u53ef\u80fd\u4f1a\u6291\u5236\u8367\u5149\u7d20\u9176\u7684\u6d3b\u6027\u3002<\/span><\/p>\n

2.<\/span> \u7ed9\u836f\u4e0e\u6210\u50cf\u65f6\u95f4\uff1a<\/span><\/strong>\u8179\u8154\u6ce8\u5c04\u5e95\u7269\u540e\uff0c\u901a\u5e38\u9700\u8981\u7b49\u5f8510-15\u5206\u949f\u8ba9\u4fe1\u53f7\u8fbe\u5230\u5cf0\u503c\u5e73\u53f0\u671f\u518d\u8fdb\u884c\u6210\u50cf\u3002\u6574\u4e2a\u7a33\u5b9a\u53d1\u5149\u7a97\u53e3\u5927\u7ea6\u6301\u7eed30-45\u5206\u949f\uff0c\u8981\u5408\u7406\u5b89\u6392\u64cd\u4f5c\u65f6\u95f4\u3002<\/span><\/p>\n

3. \u4f53\u5916\u9a8c\u8bc1\uff1a<\/strong><\/span>\u5728\u8fdb\u884c\u6602\u8d35\u7684\u52a8\u7269\u5b9e\u9a8c\u524d\uff0c\u52a1\u5fc5\u5148\u5728\u4f53\u5916\u9a8c\u8bc1\u4f60\u7684\u7ec6\u80de\u662f\u5426\u7a33\u5b9a\u8868\u8fbe\u8367\u5149\u7d20\u9176\uff0c\u5e76\u786e\u8ba4\u53d1\u5149\u5f3a\u5ea6\u4e0e\u7ec6\u80de\u6570\u91cf\u5448\u826f\u597d\u7684\u7ebf\u6027\u5173\u7cfb\uff0c\u8fd9\u80fd\u907f\u514d\u540e\u7eed\u5b9e\u9a8c\u8d70\u5f2f\u8def\u3002<\/span><\/p>\n<\/section>\n<\/section>\n

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\u9009\u5e95\u7269\u6838\u5fc3\u4e09\u539f\u5219<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n

1. \u5148\u770b\u9176\uff1a<\/span><\/strong>\u4f60\u7684\u7ec6\u80de\/\u52a8\u7269\u8868\u8fbe\u4ec0\u4e48\u9176\uff0c\u5c31\u9009\u5bf9\u5e94\u7684\u5e95\u7269\uff0c\u8fd9\u662f\u94c1\u5f8b\u3002<\/span><\/p>\n

2. \u518d\u770b\u5e94\u7528\uff1a<\/span><\/strong>\u8ffd\u6c42\u8d85\u9ad8\u7075\u654f\u5ea6\u53ef\u8003\u8651 NanoLuc\/Furimazine \u7cfb\u7edf\uff1b\u5e38\u89c4\u80bf\u7624\u3001\u5e72\u7ec6\u80de\u8ffd\u8e2a\u7528\u7ecf\u5178\u7684 Firefly Luc\/D-\u8367\u5149\u7d20\u7cfb\u7edf\u5b8c\u5168\u8db3\u591f\u3002<\/span><\/p>\n

3. \u6700\u540e\u770b\u5f62\u5f0f\uff1a<\/span><\/strong>\u4f53\u5185\u5b9e\u9a8c\u4f18\u9009 D-\u8367\u5149\u7d20\u94be\u76d0\uff0c\u6eb6\u89e3\u65b9\u4fbf\uff0c\u6027\u80fd\u7a33\u5b9a\u3002<\/span><\/p>\n<\/section>\n<\/section>\n

\n
\n
\n
\n

\u5b9e\u9a8c\u65b0\u624b\u6307\u5357<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n

\u5e38\u89c4\u5b9e\u9a8c\uff1a<\/span><\/strong>\u76f4\u63a5\u7528D-\u8367\u5149\u7d20\u94be\u76d0\uff0c\u8fd9\u662f\u6700\u7a33\u59a5\u3001\u6700\u901a\u7528\u7684\u9009\u62e9\u3002<\/span><\/p>\n

\u53cc\u6807\/\u7279\u6b8a\u7814\u7a76\uff1a<\/span><\/strong><\/span>\u6839\u636e\u4f60\u4f7f\u7528\u7684\u8367\u5149\u7d20\u9176\uff08\u5982Renilla\u6216NanoLuc\uff09\u9009\u62e9\u5bf9\u5e94\u7684\u8154\u80a0\u7d20\u6216Furimazine\u3002<\/span><\/p>\n

\u5173\u952e\u4e00\u6b65\uff1a<\/span><\/strong><\/span>\u65e0\u8bba\u9009\u54ea\u79cd\uff0c\u90fd\u5efa\u8bae\u5728\u6b63\u5f0f\u5b9e\u9a8c\u524d\u505a\u9884\u5b9e\u9a8c\uff0c\u786e\u5b9a\u6700\u4f73\u7684\u5e95\u7269\u6d53\u5ea6\u548c\u6210\u50cf\u65f6\u95f4\u70b9\u3002<\/span><\/p>\n

\"\"<\/p>\n<\/section>\n<\/section>\n

\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/p>\n

\n
\n
\n
\n

\u5b9e\u9a8c\u914d\u5957<\/span><\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\u6838\u5fc3\u9009\u54c1\u4e09\u5927\u539f\u5219<\/span><\/strong><\/span><\/section>\n
\n
    \n
  1. \u7a7f\u900f\u6df1\u5ea6\u4f18\u5148\uff1a<\/strong>\u4f18\u5148\u9009\u62e9\u8fd1\u7ea2\u5916 NIR-I\uff08700\u2013900 nm\uff09\u548c NIR-II\uff08900\u20131700 nm\uff09\u6ce2\u6bb5\uff0c\u907f\u5f00\u53ef\u89c1\u5149\uff08\u84dd\/\u7eff\/\u7ea2\uff09\uff0c\u56e0\u4e3a\u540e\u8005\u5728\u76ae\u4e0b\/\u4f53\u5185\u7684\u7a7f\u900f\u80fd\u529b\u6781\u5dee\u3002<\/span><\/li>\n
  2. \u4f53\u5185\u4f4e\u81ea\u53d1\u8367\u5149\uff1a<\/strong>\u5c0f\u9f20\u76ae\u80a4\u3001\u8840\u6db2\u3001\u5185\u810f\u5728 500\u2013650 nm \u6ce2\u6bb5\u7684\u81ea\u8eab\u8367\u5149\u6781\u5f3a\uff0c\u5e94\u5c3d\u91cf\u5c11\u7528\u7eff\u8272\u548c\u6a59\u8272\u8367\u5149\u67d3\u6599\u3002<\/span><\/li>\n
  3. \u751f\u7269\u5b89\u5168 + \u4f53\u5185\u6ede\u7559\uff1a<\/strong>\u8981\u6c42\u67d3\u6599\u4f4e\u6bd2\u6027\u3001\u4e0d\u6613\u4ee3\u8c22\u731d\u706d\u3001\u65e0\u514d\u75ab\u539f\u6027\uff0c\u540c\u65f6\u9002\u914d\u9759\u8109\u3001\u8179\u8154\u6216\u5c40\u90e8\u7ed9\u836f\u7b49\u4e0d\u540c\u9014\u5f84\u3002<\/span><\/li>\n<\/ol>\n<\/section>\n<\/section>\n<\/section>\n
    \n
    \n
    \u6309\u6ce2\u957f\u5206\u533a\u9009\u578b\uff08\u6d3b\u4f53\u4f18\u5148\u7ea7\uff09<\/span><\/strong><\/span><\/section>\n

    \u53ef\u89c1\u5149\u533a<\/span><\/strong><\/p>\n

      \n
    • \u7eff\u8272\uff08FITC\u3001Calcein\u3001GFP\uff09\uff1a\u4ec5\u9002\u7528\u4e8e\u4f53\u8868\u6d45\u5c42\u6210\u50cf\uff0c\u4f53\u5185\u6781\u6613\u88ab\u8840\u7ea2\u86cb\u767d\u5438\u6536\uff0c\u6df1\u90e8\u7ec4\u7ec7\u65e0\u6cd5\u89c2\u6d4b\u3002<\/span><\/li>\n
    • \u7ea2\u8272\uff08Cy5\u3001Cy5.5\uff09\uff1a\u6d45\u5c42\u53ef\u7528\uff0c\u4f46\u5728\u8179\u90e8\u548c\u80f8\u8154\u6210\u50cf\u65f6\u80cc\u666f\u4fe1\u53f7\u8f83\u9ad8\u3002<\/span><\/li>\n<\/ul>\n

       <\/p>\n

      \u8fd1\u7ea2\u5916\u4e00\u533a\uff08NIR-I\uff09<\/span><\/strong><\/p>\n

      \u4f53\u5185\u6210\u50cf\u9ec4\u91d1\u6ce2\u6bb5\uff0c\u7a7f\u900f\u6df1\u3001\u4eea\u5668\u901a\u7528\u3002<\/span><\/p>\n

      \n
        \n
      • ICG\uff08\u5432\u54da\u83c1\u7eff\uff09<\/span><\/strong><\/li>\n
      • Ex\/Em\uff1a780\/810 nm<\/span><\/li>\n
      • \u4f18\u70b9\uff1a\u4e34\u5e8a\u83b7\u6279\u3001\u4f53\u5185\u6bd2\u6027\u6781\u4f4e\uff0c\u662f\u8840\u7ba1\/\u6dcb\u5df4\/\u80bf\u7624\u793a\u8e2a\u7684\u9996\u9009\u3002<\/span><\/li>\n
      • \u7528\u9014\uff1a\u6d3b\u4f53\u8840\u7ba1\u6210\u50cf\u3001\u6dcb\u5df4\u5f15\u6d41\u3001\u80bf\u7624\u5bcc\u96c6\u3001\u810f\u5668\u704c\u6ce8\u3002 \u00a0\u7f3a\u70b9\uff1a\u5149\u7a33\u5b9a\u6027\u4e00\u822c\uff0c\u6613\u5feb\u901f\u4ee3\u8c22\u3002<\/span><\/li>\n<\/ul>\n<\/section>\n

         <\/p>\n

          \n
        • Cy7 \/ Cy7.5<\/span><\/strong><\/li>\n
        • Ex\/Em\uff1a750\/780 nm\uff08Cy7\uff09\u3001788\/820 nm\uff08Cy7.5\uff09<\/span><\/li>\n
        • \u4f18\u70b9\uff1a\u8367\u5149\u4eae\u5ea6\u9ad8\u3001\u4fee\u9970\u65b9\u4fbf\uff0c\u53ef\u6807\u8bb0\u86cb\u767d\/\u591a\u80bd\/\u836f\u7269\u3002<\/span><\/li>\n
        • \u7528\u9014\uff1a\u6d3b\u4f53\u9776\u5411\u80bf\u7624\u6210\u50cf\u3001\u836f\u7269\u4f53\u5185\u793a\u8e2a\u3001\u7ec6\u80de\u6d3b\u4f53\u793a\u8e2a\u3002<\/span><\/li>\n<\/ul>\n

           <\/p>\n

            \n
          • DiR\uff08\u7ec6\u80de\u819c\u4eb2\u8102\u67d3\u6599\uff09<\/span><\/strong><\/li>\n
          • Ex\/Em\uff1a750\/780 nm<\/span><\/li>\n
          • \u7528\u9014\uff1a\u6d3b\u4f53\u5e72\u7ec6\u80de\/\u514d\u75ab\u7ec6\u80de\u7684\u4f53\u5185\u8ffd\u8e2a\uff0c\u6807\u8bb0\u6d3b\u7ec6\u80de\u5e76\u89c2\u5bdf\u5176\u4f53\u5185\u8fc1\u5f99\u4e0e\u5b9a\u690d\u3002<\/span><\/li>\n<\/ul>\n

             <\/p>\n

            \u8fd1\u7ea2\u5916\u4e8c\u533a\uff08NIR-II\uff09<\/strong><\/span><\/p>\n

            \u7a7f\u900f\u6700\u6df1\u3001\u80cc\u666f\u6700\u4f4e\uff0c\u9002\u5408\u6df1\u90e8\u80bf\u7624\u3001\u8111\u90e8\u548c\u5185\u810f\u6210\u50cf\u3002<\/span><\/p>\n

              \n
            • \u4ee3\u8868\u67d3\u6599\uff1aIR-1061\u3001H\u2082O\u2082 \u63a2\u9488\u3001NIR-II \u91cf\u5b50\u70b9<\/span><\/strong><\/li>\n
            • \u4f18\u52bf\uff1a\u76ae\u4e0b >5 mm \u7ec4\u7ec7\u53ef\u6e05\u6670\u6210\u50cf\uff0c\u51e0\u4e4e\u65e0\u52a8\u7269\u81ea\u53d1\u8367\u5149\u5e72\u6270\u3002<\/span><\/li>\n
            • \u9002\u7528\uff1a\u6df1\u90e8\u5b9e\u4f53\u7624\u3001\u8111\u90e8\u539f\u4f4d\u7624\u3001\u6df1\u5c42\u810f\u5668\u793a\u8e2a\u3002<\/span><\/li>\n<\/ul>\n<\/section>\n<\/section>\n
              \n
              \n
              \n
              \n

              \u5b9e\u9a8c\u65b0\u624b\u901f\u9009\u53e3\u8bc0<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n

              \u6d45\u5c42\u89c2\u5bdf\u7528 Cy5.5\uff0c\u4f53\u5185\u6df1\u90e8\u9009 Cy7\uff1b<\/span><\/p>\n

              \u7ec6\u80de\u8ffd\u8e2a\u9760 DiR\uff0c\u8840\u7ba1\u6dcb\u5df4\u7528 ICG\uff1b<\/span><\/p>\n

              \u8ffd\u6c42\u8d85\u6e05\u6df1\u7ec4\u7ec7\uff0c\u76f4\u63a5\u4e0a NIR-II\uff1b<\/span><\/p>\n

              \u7eff\u5149\u7ea2\u5149\u522b\u5165\u4f53\uff0c\u81ea\u53d1\u8367\u5149\u5168\u5e72\u6270\u3002<\/span><\/p>\n

              \"\"<\/p>\n

              <\/section>\n

               <\/p>\n<\/section>\n<\/section>\n

              \n
              \n
              \n
              \n
              \u00a0<\/section>\n
              \n

              \u76f8\u5173\u4ea7\u54c1<\/span><\/strong><\/span><\/p>\n<\/section>\n<\/section>\n

              \n

              \u591a\u6a21\u5f0f\u5c0f\u52a8\u7269\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf<\/span><\/strong><\/p>\n

              \"\"<\/p>\n

              \u25b7<\/span> \u53cc\u6a21\u6210\u50cf<\/strong>\uff1a\u652f\u6301\u751f\u7269\u53d1\u5149\uff0b\u8367\u5149\uff0c\u4e00\u952e\u5207\u6362\u663e\u793a\u5355\u4f4d\uff08P\/S\u3001\u8f90\u4eae\u5ea6\u3001\u8367\u5149\u6548\u7387\u3001\u7070\u5ea6\u503c\uff09\u3002<\/span><\/p>\n

              \u25b6<\/span> \u8367\u5149\u6548\u7387\u76f4\u7b97\uff1a<\/strong>\u5185\u7f6e\u6fc0\u53d1\u7167\u5ea6\u6807\u5b9a\u4e0e\u4f4d\u7f6e\u6821\u6b63\uff0c\u8367\u5149\u5b9e\u9a8c\u53ef\u4ee5\u76f4\u63a5\u8f93\u51fa\u8367\u5149\u6548\u7387\uff0c\u8de8\u52a8\u7269\u3001\u8de8\u5929\u5bf9\u6bd4\u66f4\u7701\u5fc3\u3002<\/span><\/p>\n

              \u25b7<\/span> \u89c6\u91ce\u4e0e\u6e29\u63a7\uff1a<\/strong>\u7535\u52a8\u5347\u964d\u53f0\u9884\u8bbe3\u6863\u9ad8\u5ea6\uff0c\u5207\u6362\u89c6\u91ce\u5feb\uff1b\u8f7d\u6837\u53f0\u6052\u6e29\u52a0\u70ed\uff0c\u5e76\u517c\u5bb9\u5438\u5165\u9ebb\u9189\u63a5\u53e3\u3002<\/span><\/p>\n

              \u25b6<\/span> \u5b9a\u91cf\u53cb\u597d\u8f6f\u4ef6\uff1a<\/strong>ROI\u6846\u9009\u3001\u80cc\u666f\u81ea\u52a8\u6263\u9664\u5373\u53ef\u51fa\u7ed3\u679c\uff1b\u652f\u6301\u6279\u91cf\u5206\u6790\u3001\u53c2\u6570\u6a21\u677f\u590d\u7528\uff0c\u539f\u59cb\u6570\u636e\u548c\u62a5\u544a\u56fe\u53ef\u540c\u65f6\u5bfc\u51fa\u3002<\/span><\/p>\n

              \u25b7<\/span> \u5149\u5b66\u6269\u5c55\uff1a<\/strong>\u57fa\u7840 400-800nm \u6807\u914d\uff0c\u591a\u901a\u9053\u6ee4\u7247\u53ef\u6269\u5c55\u5230\u8fd1\u7ea2\u5916\uff08900-1700nm\uff09\uff0c\u8fd8\u53ef\u52a0\u88c5X\u5149\u6a21\u5757\uff0c\u6ee1\u8db3\u5149\u8c31\u5206\u79bb\u548c\u591a\u8272\u6210\u50cf\u9700\u6c42\u3002<\/span><\/p>\n

              \u25b6<\/span> \u81ea\u52a8\u8bb0\u5f55\uff1a<\/strong>\u66dd\u5149\u3001\u589e\u76ca\u3001\u6ee4\u7247\u548c\u51e0\u4f55\u4fe1\u606f\u90fd\u4f1a\u81ea\u52a8\u4fdd\u5b58\uff0c\u4fdd\u8bc1\u5b9e\u9a8c\u53e3\u5f84\u4e00\u81f4\uff0c\u590d\u73b0\u5b9e\u9a8c\u66f4\u5b89\u5fc3\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

              \u00a0<\/section>\n

               <\/p>\n

               <\/p>\n

               <\/p>\n

              \u90e8\u5206\u53c2\u8003\u6587\u732e\uff1a<\/span><\/strong>[1]Coste, A., Oktay, M. H., Condeelis, J. S., & Entenberg, D. (2020). Intravital imaging techniques for biomedical and clinical research.\u00a0Cytometry Part A,\u00a097(5), 448\u2013457.\u00a0https:\/\/doi.org\/10.1002\/cyto.a.23963<\/span>[2] Su, Y., Walker, J. R., Park, Y., Smith, T. P., Liu, L. X., Hall, M. P., Labanieh, L., Hurst, R., Wang, D. C., Encell, L. P., Kim, N., Zhang, F., Kay, M. A., Casey, K. M., Majzner, R. G., Cochran, J. R., Mackall, C. L., Kirkland, T. A., & Lin, M. Z. (2020). Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals.\u00a0Nature Methods,\u00a017(8), 852\u2013860.\u00a0https:\/\/doi.org\/10.1038\/s41592-020-0889-6<\/span><\/p>\n

              [3]Wu, Y., Hao, C., Gao, C., Hageman, M., Lee, S., Kirkland, T. A., Gray, N. S., Su, Y., & Lin, M. Z. (2026). Abstract 2129: Pharmacodynamics of Akt drugs revealed by a kinase-modulated bioluminescent indicator with BBB-permeable substrate. Cancer Research, 86(7_Supplement).\u00a0https:\/\/doi.org\/10.1158\/1538-7445.AM2026-2129<\/span><\/p>\n

              [4]Fichtner, J., Wagenh\u00e4user, Y., S\u00e1rosi, M., Stolte, M., & W\u00fcrthner, F. (2026). Fluorescent merocyanines: from fundamental properties to applications as molecular probes, in bioimaging and as emissive dye aggregates. Chemical Society Reviews.\u00a0https:\/\/doi.org\/10.1039\/D6CS00194G<\/span><\/p>\n

              [5]Mu, H., Miki, K., & Ohe, K. (2021). pH-Activatable cyanine dyes for selective tumor imaging using near-infrared fluorescence and photoacoustic modalities. ACS Sensors, 6(1), 123\u2013129.\u00a0https:\/\/doi.org\/10.1021\/acssensors.0c01926<\/span><\/p>\n

              [6]Lu, Y. J., Hu, D. P., Zhang, K., Wong, W. L., & Chow, C. F. (2016). New pyridinium-based fluorescent dyes: A comparison of symmetry and side-group effects on G-Quadruplex DNA binding selectivity and application in live cell imaging. Biosensors and Bioelectronics, 81, 373\u2013381.\u00a0https:\/\/doi.org\/10.1016\/j.bios.2016.03.012<\/span><\/p>\n

              \u90e8\u5206\u63d2\u56fe\u6765\u6e90\uff1a\u5c0f\u7ea2\u4e66\u53f7262802942\u3001\u5c0f\u7ea2\u4e66\u53f72588520423<\/span><\/section>\n<\/section>\n","protected":false},"excerpt":{"rendered":"

              \u4ec0\u4e48\u662f\u6d3b\u4f53\u6210\u50cf 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