{"id":7984,"date":"2025-12-22T15:01:40","date_gmt":"2025-12-22T07:01:40","guid":{"rendered":"http:\/\/www.bio-oi.com\/?p=7984"},"modified":"2026-03-19T13:54:26","modified_gmt":"2026-03-19T05:54:26","slug":"%e6%96%87%e7%8c%ae%e5%af%bc%e8%af%bb-laser%e7%b3%bb%e5%88%97%e6%b4%bb%e4%bd%93%e6%88%90%e5%83%8f%e7%b3%bb%e7%bb%9f%e5%8a%a9%e5%8a%9b%e8%bd%af%e6%9e%a3%e7%8c%95%e7%8c%b4%e6%a1%83%e8%8a%b1%e9%9d%92","status":"publish","type":"post","link":"https:\/\/www.bio-oi.com\/en\/2025\/12\/22\/%e6%96%87%e7%8c%ae%e5%af%bc%e8%af%bb-laser%e7%b3%bb%e5%88%97%e6%b4%bb%e4%bd%93%e6%88%90%e5%83%8f%e7%b3%bb%e7%bb%9f%e5%8a%a9%e5%8a%9b%e8%bd%af%e6%9e%a3%e7%8c%95%e7%8c%b4%e6%a1%83%e8%8a%b1%e9%9d%92\/","title":{"rendered":"\u6587\u732e\u5bfc\u8bfb | LASER\u7cfb\u5217\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf\u52a9\u529b\u8f6f\u67a3\u7315\u7334\u6843\u82b1\u9752\u7d20\u8c03\u63a7\u673a\u5236\u89e3\u6790"},"content":{"rendered":"
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\u56fd\u9645\u5b66\u672f\u671f\u520a\u300aMolecular Horticulture\u300b\u53d1\u8868\u4e86\u4e00\u9879\u5173\u4e8e\u8f6f\u67a3\u7315\u7334\u6843\uff08Actinidia arguta\uff09\u82b1\u9752\u7d20\u8c03\u63a7\u673a\u5236\u7684\u91cd\u8981\u7814\u7a76\u3002\u4f5c\u4e3a\u4e00\u79cd\u5916\u89c2\u8bf1\u4eba\u3001\u679c\u76ae\u53ef\u98df\u4e14\u8425\u517b\u4e30\u5bcc\u7684\u65b0\u5174\u6c34\u679c\uff0c\u8f6f\u67a3\u7315\u7334\u6843\u5728\u5546\u4e1a\u5316\u683d\u57f9\u4e2d\u5374\u9762\u4e34\u7ea2\u8272\u6027\u72b6\u4e0d\u7a33\u5b9a<\/strong>\u7684\u4ea7\u4e1a\u74f6\u9888\uff0c\u800c\u4f20\u7edf\u80b2\u79cd\u5468\u671f\u957f\u3001\u6548\u7387\u6709\u9650\uff0c\u4e9f\u9700\u4ece\u5206\u5b50\u5c42\u9762\u89e3\u6790\u8272\u6cfd\u5f62\u6210\u673a\u5236\u5e76\u5f00\u53d1\u80b2\u79cd\u6807\u8bb0\u3002\u5728\u6b64\u80cc\u666f\u4e0b\uff0c\u4e2d\u56fd\u519c\u4e1a\u79d1\u5b66\u9662\u90d1\u5dde\u679c\u6811\u7814\u7a76\u6240\u9f50\u79c0\u5a1f\u7814\u7a76\u5458\u56e2\u961f\u5b8c\u6210\u4e86\u9996\u4e2a\u5168\u7ea2\u8089\u8f6f\u67a3\u7315\u7334\u6843\u201c\u5929\u6e90\u7ea2\u201d\u7684\u67d3\u8272\u4f53\u7ea7\u522b\u57fa\u56e0\u7ec4\u7ec4\u88c5\uff0c\u7cfb\u7edf\u63ed\u793a\u4e86\u82b1\u9752\u7d20\u79ef\u7d2f\u7684\u8c03\u63a7\u8def\u5f84\u3002\u7814\u7a76\u53d1\u73b0\uff0c\u4e00\u4e2a\u540d\u4e3aAaBEE1\u7684bHLH\u8f6c\u5f55\u56e0\u5b50\u80fd\u591f\u76f4\u63a5\u7ed3\u5408\u82b1\u9752\u7d20\u5173\u952e\u57fa\u56e0AaLDOX\u7684\u542f\u52a8\u5b50<\/strong>\uff0c\u4ece\u800c\u8d1f\u5411\u8c03\u63a7<\/strong>\u5176\u8868\u8fbe\uff1b\u8fdb\u4e00\u6b65\u7814\u7a76\u9274\u5b9a\u51fa\u542f\u52a8\u5b50\u533a\u4e00\u4e2a\u4e0e\u679c\u8089\u989c\u8272\u7d27\u5bc6\u5173\u8054\u768429-bp indel\u53d8\u5f02<\/strong>\uff0c\u5e76\u6210\u529f\u5c06\u5176\u5f00\u53d1\u4e3a\u53ef\u7528\u4e8e\u65e9\u671f\u8f85\u52a9\u9009\u62e9\u7684\u5206\u5b50\u6807\u8bb0\u3002\u8be5\u7814\u7a76\u4e0d\u4ec5\u9610\u660e\u4e86\u7ea2\u8089\u8f6f\u67a3\u7315\u7334\u6843\u7740\u8272\u7684\u5206\u5b50\u5f00\u5173\uff0c\u4e5f\u4e3a\u63a8\u8fdb\u7315\u7334\u6843\u8272\u6cfd\u80b2\u79cd\u63d0\u4f9b\u4e86\u5173\u952e\u57fa\u56e0\u8d44\u6e90\u4e0e\u5b9e\u7528\u5de5\u5177\u3002<\/span><\/p>\n

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\u7814\u7a76\u4e3b\u7ebf<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u7814\u7a76\u56f4\u7ed5\u201c\u7ea2\u8089\u5f62\u6210\u673a\u5236\u201d\u5c55\u5f00\uff0c\u6784\u5efa\u4e86\u4e00\u5957\u4ece\u57fa\u56e0\u7ec4\u5230\u8868\u578b\u7684\u5b8c\u6574\u7814\u7a76\u4f53\u7cfb\uff1a<\/span><\/p>\n

\u6750\u6599\u7cfb\u7edf\u6784\u5efa\uff1a<\/span><\/strong><\/span><\/p>\n

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  • \u4ee5\u5168\u7ea2\u578b\u54c1\u79cd\u201c\u5929\u6e90\u7ea2\u201d\u4e3a\u6750\u6599\u5b8c\u6210\u57fa\u56e0\u7ec4\u7ec4\u88c5\uff1b<\/span><\/li>\n
  • \u9009\u53d6\u7ea2\u8089\uff08ZHB\uff09\u4e0e\u7eff\u8089\uff08ZLB\uff09\u54c1\u7cfb\uff0c\u8fdb\u884c\u591a\u7ec4\u7ec7\u3001\u591a\u53d1\u80b2\u9636\u6bb5\u7684\u8f6c\u5f55\u7ec4\u6bd4\u8f83\uff1b<\/span><\/li>\n
  • \u5229\u7528\u70df\u8349\u3001\u756a\u8304\u3001\u62df\u5357\u82a5\u53ca\u7315\u7334\u6843\u679c\u5b9e\u7b49\u591a\u79cd\u9057\u4f20\u8f6c\u5316\u4f53\u7cfb\u8fdb\u884c\u529f\u80fd\u9a8c\u8bc1\u3002<\/span><\/li>\n<\/ul>\n

    \u6280\u672f\u8def\u5f84\u8bbe\u8ba1\uff1a<\/span><\/strong><\/span><\/p>\n

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    • \u901a\u8fc7\u6bd4\u8f83\u57fa\u56e0\u7ec4\u5206\u6790\u63a2\u8ba8\u7269\u79cd\u7279\u5f02\u6027\u72b6\u5f62\u6210\u7684\u8fdb\u5316\u57fa\u7840\uff1b<\/span><\/li>\n
    • \u57fa\u4e8eRNA-seq\u7b5b\u9009\u5019\u9009\u57fa\u56e0\uff0c\u7ed3\u5408DAP-seq\u5bfb\u627e\u9776\u70b9\uff1b<\/span><\/li>\n
    • \u8fd0\u7528Y1H\u3001EMSA\u3001ChIP-qPCR\u3001LUC\u7b49\u591a\u79cd\u6280\u672f\u9a8c\u8bc1\u8f6c\u5f55\u56e0\u5b50\u4e0e\u542f\u52a8\u5b50\u7684\u4e92\u4f5c\u5173\u7cfb\uff1b<\/span><\/li>\n
    • \n

      \u514b\u9686AaLDOX\u542f\u52a8\u5b50\uff0c\u5206\u6790\u5176\u53d8\u5f02\u4e0e\u989c\u8272\u8868\u578b\u7684\u5173\u8054\uff0c\u5f00\u53d1\u53ef\u7528\u4e8e\u80b2\u79cd\u7b5b\u9009\u7684 indel \u6807\u8bb0\u3002<\/span><\/p>\n<\/li>\n<\/ul>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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      \u53cc\u8367\u5149\u7d20\u9176\u62a5\u544a\u57fa\u56e0\u6210\u50cf\uff08LUC Assay\uff09<\/span><\/strong><\/span><\/p>\n

      \u7814\u7a76\u56e2\u961f\u5c06AaBEE1\u6548\u5e94\u8f7d\u4f53<\/strong>\u4e0eAaLDOXpro-LUC\u62a5\u544a\u8f7d\u4f53<\/strong>\u5171\u8f6c\u67d3\u672c\u6c0f\u70df\u8349\u53f6\u7247\uff0c\u57f9\u517b3\u5929\u540e\u4f7f\u7528LASER\u7cfb\u5217\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf<\/strong><\/span>\u8fdb\u884c\u8367\u5149\u4fe1\u53f7\u91c7\u96c6\u3002\u7cfb\u7edf\u914d\u7f6e\u9ad8\u7075\u654f\u5ea6\u81ea\u52a8\u5bf9\u7126\u955c\u5934\uff0c\u6709\u6548\u6355\u6349Firefly\u4e0eRenilla\u8367\u5149\u7d20\u9176\u4fe1\u53f7\uff0c\u5e76\u901a\u8fc7\u8f6f\u4ef6\u8ba1\u7b97LUC\/REN\u6bd4\u503c\uff0c\u5b9a\u91cf\u5206\u6790\u542f\u52a8\u5b50\u6d3b\u6027\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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      \u76f8\u5173\u53d1\u73b0<\/strong><\/span><\/p>\n

      AaBEE1\u6291\u5236AaLDOX\u542f\u52a8\u5b50\u6d3b\u6027<\/span><\/strong><\/span><\/p>\n

      LUC\u6210\u50cf\u663e\u793a\uff0c\u5171\u8f6cAaBEE1\u4e0eAaLDOXpro\u7684\u53f6\u7247\u8367\u5149\u4fe1\u53f7\u663e\u8457\u5f31\u4e8e\u5bf9\u7167\uff0c\u8bc1\u5b9eAaBEE1\u5bf9AaLDOX\u542f\u52a8\u5b50\u5177\u6709\u6291\u5236\u4f5c\u7528\u3002<\/span><\/p>\n

      \"\"<\/p>\n<\/section>\n<\/section>\n

      \n
      Fig 5d-e: LUC\/REN\u6bd4\u503c\u4e0e\u8367\u5149\u6210\u50cf\u663e\u793aAaBEE1\u5bf9AaLDOX\u542f\u52a8\u5b50\u7684\u6291\u5236\u6548\u679c<\/span><\/section>\n<\/section>\n
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      29-bp indel\u51b3\u5b9a\u542f\u52a8\u5b50\u6d3b\u6027\u5f3a\u5f31<\/span><\/strong><\/span><\/p>\n

      \u8fdb\u4e00\u6b65\u6bd4\u8f83\u7ea2\/\u7eff\u54c1\u79cd\u4e2dAaLDOX\u542f\u52a8\u5b50\u6d3b\u6027\uff0c\u53d1\u73b029-bp\u63d2\u5165\u578b\u542f\u52a8\u5b50\u6d3b\u6027\u663e\u8457\u9ad8\u4e8e\u7f3a\u5931\u578b\uff0c\u89e3\u91ca\u4e86\u7ea2\u8089\u54c1\u79cd\u4e2dAaLDOX\u9ad8\u8868\u8fbe\u7684\u539f\u56e0\u3002<\/span><\/p>\n

      \"\"<\/p>\n<\/section>\n<\/section>\n

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      Fig 7c-d: \u7ea2\/\u7eff\u54c1\u79cdAaLDOX\u542f\u52a8\u5b50\u6d3b\u6027\u6bd4\u8f83<\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
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      \u5b9e\u9a8c\u914d\u5957<\/span><\/strong><\/p>\n

      \u4e3a\u4f55\u9009\u62e9LASER\u7cfb\u5217\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf\u8fdb\u884cLUC\u6d3b\u4f53\u6210\u50cf\uff1f<\/span><\/strong><\/p>\n

      \u672c\u7814\u7a76\u4e2d\u7684\u53cc\u8367\u5149\u7d20\u9176\u62a5\u544a\u5b9e\u9a8c\u5bf9\u6d3b\u4f53\u6210\u50cf\u8bbe\u5907\u63d0\u51fa\u4e86\u6838\u5fc3\u8981\u6c42\uff1a\u9700\u5177\u5907\u9ad8\u7075\u654f\u5ea6\u4ee5\u6355\u83b7\u5fae\u5f31\u4fe1\u53f7\uff0c\u5e76\u517c\u5bb9\u591a\u6837\u672c\u7c7b\u578b\u4ee5\u9002\u5e94\u690d\u7269\u5b9e\u9a8c\u573a\u666f\u3002<\/strong><\/span><\/span><\/p>\n

      LASER\u7cfb\u5217\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf\u7684\u8bbe\u5907\u8981\u70b9\u4e0e\u6b64\u9ad8\u5ea6\u5951\u5408\uff1a<\/span><\/p>\n

      \u25b7<\/span>\u00a0\u9ad8\u7075\u654f\u5ea6\u6df1\u51b7\u76f8\u673a\u4e0e\u5927\u53e3\u5f84\u955c\u5934\uff1a<\/strong>\u652f\u6301\u5fae\u5f31\u8367\u5149\u7d20\u9176\u4fe1\u53f7\u7684\u5feb\u901f\u81ea\u52a8\u91c7\u96c6\uff0c\u786e\u4fdd\u542f\u52a8\u5b50\u6d3b\u6027\u7684\u7ec6\u5fae\u5dee\u5f02\u5f97\u4ee5\u53ef\u9760\u91cf\u5316\uff1b\u4e3a\u6570\u636e\u6807\u51c6\u5316\u548c\u6bd4\u7387\u8ba1\u7b97\u63d0\u4f9b\u4fbf\u5229\uff1b<\/span><\/p>\n

      \u25b7<\/span>\u00a0\u591a\u529f\u80fd\u96c6\u6210\u6837\u54c1\u53f0\uff1a<\/strong>\u53ef\u517c\u5bb9\u6d3b\u4f53\u690d\u7269\u3001\u57f9\u517b\u76bf\u3001\u591a\u5b54\u677f\u7b49\u591a\u79cd\u6837\u672c\uff0c\u5b8c\u7f8e\u9002\u914d\u4ece\u53f6\u7247\u4fb5\u67d3\u5230\u591a\u6837\u672c\u7b5b\u9009\u7684\u5b9e\u9a8c\u6d41\u7a0b\u3002<\/span><\/p>\n

      LASER\u7cfb\u5217\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf<\/strong><\/span>\u5728\u672c\u7814\u7a76\u4e2d\u6210\u529f\u6355\u6349\u5230AaBEE1\u5bf9AaLDOX\u542f\u52a8\u5b50\u7684\u6291\u5236\u6548\u5e94\uff0c\u4e3a\u201c\u8f6c\u5f55\u56e0\u5b50\u2014\u542f\u52a8\u5b50\u201d\u4e92\u4f5c\u63d0\u4f9b\u4e86\u76f4\u89c2\u8bc1\u636e\uff0c\u6210\u4e3a\u57fa\u56e0\u529f\u80fd\u9a8c\u8bc1\u94fe\u6761\u4e2d\u5173\u952e\u4e00\u73af\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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      \u5c0f\u7ed3<\/span><\/strong><\/span><\/p>\n

      \u672c\u7814\u7a76\u4ece\u57fa\u56e0\u7ec4\u51fa\u53d1\uff0c\u7cfb\u7edf\u89e3\u6790\u4e86\u8f6f\u67a3\u7315\u7334\u6843\u82b1\u9752\u7d20\u5408\u6210\u7684\u8d1f\u8c03\u63a7\u673a\u5236\uff0c\u4e0d\u4ec5\u4e3a\u7406\u89e3\u679c\u5b9e\u7740\u8272\u63d0\u4f9b\u4e86\u65b0\u89c6\u89d2\uff0c\u4e5f\u4e3a\u5206\u5b50\u6807\u8bb0\u8f85\u52a9\u80b2\u79cd\u5960\u5b9a\u4e86\u7406\u8bba\u57fa\u7840\u3002LASER\u7cfb\u5217\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf<\/strong>\u5728\u542f\u52a8\u5b50\u6d3b\u6027\u5206\u6790\u4e2d\u7684\u6210\u529f\u5e94\u7528\uff0c\u518d\u6b21\u8bc1\u660e\u4e86\u9ad8\u6027\u80fd\u6210\u50cf\u8bbe\u5907\u5728\u690d\u7269\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u7684\u4ef7\u503c\u2014\u2014\u8ba9\u4e0d\u53ef\u89c1\u7684\u57fa\u56e0\u8c03\u63a7\uff0c\u53d8\u5f97\u6e05\u6670\u53ef\u89c1<\/strong>\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

      \u53c2\u8003\u6587\u732e\uff1a<\/strong>Li Y, Song Z, Zhan X, et al. Chromosome-level genome assembly assists in dissecting mechanism of anthocyanin regulation in kiwifruit (Actinidia arguta). Mol Hortic. 2025;5:18. https:\/\/doi.org\/10.1186\/s43897-024-00139-7<\/span><\/p>\n<\/section>\n

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      \u63a8\u8350\u8bbe\u5907<\/span><\/strong><\/span><\/p>\n

      LASER\u7cfb\u5217\u52a8\u690d\u7269\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf<\/span><\/strong><\/p>\n

      \u25b7<\/span>\u00a0\u79d1\u7814\u7ea7\u6df1\u5236\u51b7\u76f8\u673a\u642d\u914d\u5927\u5149\u5708\u81ea\u52a8\u5bf9\u7126\u955c\u5934\uff0c\u6355\u83b7\u6781\u5fae\u5f31\u7684\u5149\u4fe1\u53f7\uff0c\u5b9e\u73b0\u6700\u9ad8\u4fe1\u566a\u6bd4\u7684\u6210\u50cf\u3002<\/span><\/p>\n

      \u25b7<\/span>\u00a0\u5cf0\u503cQE\u53ef\u8fbe\u7ea695%\uff0c\u5728\u53ef\u89c1-\u8fd1\u7ea2\u5916\u6ce2\u6bb5\u90fd\u80fd\u9ad8\u6548\u6536\u5149\uff0c\u6697\u6761\u5e26\u4fe1\u53f7\u4e5f\u4e0d\u6d41\u5931\u3002<\/span><\/p>\n

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      \"\"<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

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\u514b\u9686AaLDOX\u542f\u52a8\u5b50\uff0c\u5206\u6790\u5176\u53d8\u5f02\u4e0e\u989c\u8272\u8868\u578b\u7684\u5173\u8054\uff0c\u5f00\u53d1\u53ef\u7528\u4e8e\u80b2\u79cd\u7b5b\u9009\u7684 indel \u6807\u8bb0\u3002 \u53cc\u8367\u5149\u7d20\u9176\u62a5\u544a\u57fa\u56e0\u6210\u50cf\uff08LUC Assay\uff09 \u7814\u7a76\u56e2\u961f\u5c06AaBEE1\u6548\u5e94\u8f7d\u4f53\u4e0eAaLDOXpro-LUC\u62a5\u544a\u8f7d\u4f53\u5171\u8f6c\u67d3\u672c\u6c0f\u70df\u8349\u53f6\u7247\uff0c\u57f9\u517b3\u5929\u540e\u4f7f\u7528LASER\u7cfb\u5217\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf\u8fdb\u884c\u8367\u5149\u4fe1\u53f7\u91c7\u96c6\u3002\u7cfb\u7edf\u914d\u7f6e\u9ad8\u7075\u654f\u5ea6\u81ea\u52a8\u5bf9\u7126\u955c\u5934\uff0c\u6709\u6548\u6355\u6349Firefly\u4e0eRenilla\u8367\u5149\u7d20\u9176\u4fe1\u53f7\uff0c\u5e76\u901a\u8fc7\u8f6f\u4ef6\u8ba1\u7b97LUC\/REN\u6bd4\u503c\uff0c\u5b9a\u91cf\u5206\u6790\u542f\u52a8\u5b50\u6d3b\u6027\u3002 \u76f8\u5173\u53d1\u73b0 AaBEE1\u6291\u5236AaLDOX\u542f\u52a8\u5b50\u6d3b\u6027 LUC\u6210\u50cf\u663e\u793a\uff0c\u5171\u8f6cAaBEE1\u4e0eAaLDOXpro\u7684\u53f6\u7247\u8367\u5149\u4fe1\u53f7\u663e\u8457\u5f31\u4e8e\u5bf9\u7167\uff0c\u8bc1\u5b9eAaBEE1\u5bf9AaLDOX\u542f\u52a8\u5b50\u5177\u6709\u6291\u5236\u4f5c\u7528\u3002 Fig 5d-e: LUC\/REN\u6bd4\u503c\u4e0e\u8367\u5149\u6210\u50cf\u663e\u793aAaBEE1\u5bf9AaLDOX\u542f\u52a8\u5b50\u7684\u6291\u5236\u6548\u679c 29-bp indel\u51b3\u5b9a\u542f\u52a8\u5b50\u6d3b\u6027\u5f3a\u5f31 \u8fdb\u4e00\u6b65\u6bd4\u8f83\u7ea2\/\u7eff\u54c1\u79cd\u4e2dAaLDOX\u542f\u52a8\u5b50\u6d3b\u6027\uff0c\u53d1\u73b029-bp\u63d2\u5165\u578b\u542f\u52a8\u5b50\u6d3b\u6027\u663e\u8457\u9ad8\u4e8e\u7f3a\u5931\u578b\uff0c\u89e3\u91ca\u4e86\u7ea2\u8089\u54c1\u79cd\u4e2dAaLDOX\u9ad8\u8868\u8fbe\u7684\u539f\u56e0\u3002 Fig 7c-d: \u7ea2\/\u7eff\u54c1\u79cdAaLDOX\u542f\u52a8\u5b50\u6d3b\u6027\u6bd4\u8f83 \u5b9e\u9a8c\u914d\u5957 \u4e3a\u4f55\u9009\u62e9LASER\u7cfb\u5217\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf\u8fdb\u884cLUC\u6d3b\u4f53\u6210\u50cf\uff1f 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