{"id":7906,"date":"2025-09-22T14:54:35","date_gmt":"2025-09-22T06:54:35","guid":{"rendered":"http:\/\/www.bio-oi.com\/?p=7906"},"modified":"2026-08-13T13:52:12","modified_gmt":"2026-08-13T05:52:12","slug":"%e7%94%9f%e7%89%a9%e5%8f%91%e5%85%89-vs-%e8%8d%a7%e5%85%89%e6%88%90%e5%83%8f%ef%bc%8c%e6%80%8e%e4%b9%88%e9%80%89%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/www.bio-oi.com\/en\/2025\/09\/22\/%e7%94%9f%e7%89%a9%e5%8f%91%e5%85%89-vs-%e8%8d%a7%e5%85%89%e6%88%90%e5%83%8f%ef%bc%8c%e6%80%8e%e4%b9%88%e9%80%89%ef%bc%9f\/","title":{"rendered":"\u751f\u7269\u53d1\u5149 vs \u8367\u5149\u6210\u50cf\uff0c\u600e\u4e48\u9009\uff1f"},"content":{"rendered":"
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1980\u5e74\u4ee3\uff0c\u5206\u5b50\u751f\u7269\u5b66\u5c06\u8be5\u4f53\u7cfb\u7528\u4e8e\u57fa\u56e0\u7814\u7a76\uff1aOw \u5c06\u8367\u5149\u7d20\u9176\u57fa\u56e0\u5bfc\u5165\u690d\u7269\uff0cde\u202fWet \u5e94\u7528\u4e8e\u54fa\u4e73\u52a8\u7269\uff0c\u786e\u7acb\u5176\u4e3a\u8de8\u7269\u79cd\u3001\u9ad8\u7075\u654f\u3001\u53ef\u5b9a\u91cf\u7684\u62a5\u544a\u57fa\u56e0\uff1b\u901a\u8fc7\u4f53\u5185\u6ce8\u5c04\u5e95\u7269\u5373\u53ef\u201c\u70b9\u4eae\u201d\u76ee\u6807\u7ec6\u80de\u3002<\/span><\/p>\n

1990\u5e74\u4ee3\u4e2d\u671f\uff0c\u8fd9\u4e00\u4f53\u7cfb\u9996\u6b21\u7528\u4e8e\u6d3b\u4f53\u6210\u50cf\u3002Christopher Contag\u56e2\u961f\u5229\u7528\u7ec6\u83cc\u53d1\u5149\u57fa\u56e0\u5728\u5c0f\u9f20\u4f53\u5185\u5b9e\u73b0\u975e\u4fb5\u5165\u6210\u50cf\uff1b1997\u5e74\uff0c\u4ed6\u4eec\u8fdb\u4e00\u6b65\u5c06\u8424\u706b\u866b\u4f53\u7cfb\u7528\u4e8e\u54fa\u4e73\u52a8\u7269\uff0c\u5b9e\u73b0\u4e86\u6d3b\u4f53\u57fa\u56e0\u8868\u8fbe\u53ef\u89c6\u5316\u3002\u8fd9\u4e00\u7a81\u7834\u4f7f\u7814\u7a76\u8005\u80fd\u591f\u52a8\u6001\u89c2\u5bdf\u80bf\u7624\u751f\u957f\u3001\u57fa\u56e0\u8868\u8fbe\u53ca\u836f\u7269\u4f5c\u7528\uff0c\u5927\u5e45\u62d3\u5c55\u4e86\u751f\u7269\u533b\u5b66\u7814\u7a76\u624b\u6bb5\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

\u56fe1.\u8424\u706b\u866b\u751f\u7269\u53d1\u5149\uff08\u6765\u6e90\u65b0\u6d6ahttps:\/\/k.sina.com.cn\/article_3857243723_pe5e8de4b0010057t7.html\uff09<\/span><\/p>\n

\u56fe2.\u8424\u706b\u866b\u8367\u5149\u7d20\u9176\u5316\u5b66\u673a\u7406\u793a\u610f\uff08\u6765\u6e90\u77e5\u4e4ehttps:\/\/zhuanlan.zhihu.com\/p\/670401009\uff09<\/span><\/p>\n

\u56fe3.\u5c0f\u9f20\u4f53\u5185\u751f\u7269\u53d1\u5149\u6210\u50cf\uff08\u6765\u6e90https:\/\/blog.csdn.net\/Bio12345\/article\/details\/111992856\uff09<\/span><\/p>\n

 <\/p>\n

\u8367\u5149\u6210\u50cf\uff1a\u4ece\u6c34\u6bcd\u5230\u591a\u8272\u8367\u5149<\/span><\/strong><\/span><\/p>\n

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\u9664\u57fa\u56e0\u6807\u8bb0\u5916\uff0c\u8fd8\u5f00\u53d1\u4e86\u591a\u79cd\u5c0f\u5206\u5b50\u8367\u5149\u67d3\u6599\uff08\u5982 Cy5\u3001Cy7\u3001ICG \u7b49\uff09\uff0c\u53ef\u4e0e\u7279\u5b9a\u5206\u5b50\u7ed3\u5408\uff0c\u7ecf\u5916\u90e8\u5149\u6e90\u6fc0\u53d1\u5b9e\u73b0\u6210\u50cf\u3002\u6210\u50cf\u6ce2\u6bb5\u4e5f\u6269\u5c55\u81f3\u8fd1\u7ea2\u5916 NIR-I\uff08650\u2013900\u202fnm\uff09\u548c NIR-II\uff081000\u20131700\u202fnm\uff09\uff0c\u4ee5\u63d0\u9ad8\u7ec4\u7ec7\u7a7f\u900f\u548c\u4fe1\u566a\u6bd4\uff0c\u652f\u6301\u66f4\u6df1\u90e8\u6210\u50cf\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

\u56fe1.\u8367\u5149\u7684 Jablonski \u80fd\u7ea7\u56fe\uff08\u6765\u6e90\u77e5\u4e4ehttps:\/\/zhuanlan.zhihu.com\/p\/477984559\uff09<\/span><\/p>\n

\u56fe2.\u843d\u5c04\u8367\u5149\u663e\u5fae\/\u6210\u50cf\u5149\u8def\uff08\u6765\u6e90\u7269\u7406\u5b66\u62a5https:\/\/wulixb.iphy.ac.cn\/cn\/article\/doi\/10.7498\/aps.69.20200908\uff09<\/span><\/p>\n

\u56fe3.\u8fd1\u7ea2\u5916\u8367\u5149\u5c0f\u9f20\u6210\u50cf\uff08\u6765\u6e90\u641c\u72d0https:\/\/www.sohu.com\/a\/617789167_120463448\uff09<\/span><\/p>\n

\u56fe4.\u534e\u4eba\u8bfa\u8d1d\u5c14\u5956\u83b7\u5f97\u8005Roger Tsien\uff08\u94b1\u6c38\u5065\uff09\uff08\u6765\u6e90\u767e\u5ea6\u8bcd\u6761\uff09<\/span><\/p>\n

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 <\/p>\n

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\u751f\u7269\u53d1\u5149\uff1a<\/strong>\u4f9d\u8d56\u8367\u5149\u7d20\u9176+\u5e95\u7269\u7684\u5316\u5b66\u53cd\u5e94\uff0c\u5728\u4f53\u5185\u76f4\u63a5\u4ea7\u751f\u5149\u5b50\u3002\u65e0\u9700\u5916\u90e8\u5149\u6e90\uff0c\u80cc\u666f\u566a\u58f0\u6781\u4f4e\uff0c\u4fe1\u566a\u6bd4\u9ad8\uff0c\u5c24\u5176\u9002\u5408\u68c0\u6d4b\u4f4e\u6c34\u5e73\u7684\u57fa\u56e0\u8868\u8fbe\u6216\u5c11\u91cf\u7ec6\u80de\u53d8\u5316\u3002\u5e95\u7269\u6ce8\u5c04\u673a\u5236\u8fd8\u80fd\u8ba9\u53d1\u5149\u7684\u65f6\u95f4\u548c\u90e8\u4f4d\u53ef\u63a7\u3002<\/span><\/p>\n

\u8367\u5149\u6210\u50cf\uff1a<\/strong>\u4f9d\u8d56\u5916\u90e8\u5149\u6e90\u6fc0\u53d1\uff0c\u8ba9\u8367\u5149\u5206\u5b50\u7535\u5b50\u8dc3\u8fc1\u5e76\u56de\u5230\u57fa\u6001\u65f6\u91ca\u653e\u5149\u5b50\uff0c\u4fe1\u53f7\u4eae\u5ea6\u66f4\u9ad8\uff0c\u80fd\u591f\u8f7b\u677e\u5b9e\u73b0\u591a\u989c\u8272\u3001\u591a\u9776\u70b9\u540c\u6b65\u6210\u50cf\u3002<\/span><\/p>\n

 <\/p>\n

 <\/p>\n

\u5b83\u4eec\u5404\u81ea\u7684\u4f18\u7f3a\u70b9\u662f\u4ec0\u4e48\uff1f<\/span><\/strong><\/span><\/p>\n

\u751f\u7269\u53d1\u5149\uff1a<\/span><\/strong><\/p>\n

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\u8367\u5149\u6210\u50cf\uff1a<\/span><\/strong><\/p>\n

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 <\/p>\n

 <\/p>\n

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 <\/p>\n

\u4ec0\u4e48\u65f6\u5019\u4f18\u5148\u9009\u62e9\u8367\u5149\u6210\u50cf\uff1f<\/span><\/strong><\/p>\n

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 <\/p>\n

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\"\"<\/span><\/p>\n

\u56fe\u8868.\u4e24\u79cd\u6210\u50cf\u6a21\u5f0f\u5bf9\u6bd4<\/span><\/p>\n

 <\/p>\n

 <\/p>\n

\u76f8\u5173\u4ea7\u54c1<\/span><\/strong><\/span><\/p>\n

\u4e3a\u4e86\u5145\u5206\u5229\u7528\u751f\u7269\u53d1\u5149\u4e0e\u8367\u5149\u6210\u50cf\u5404\u81ea\u7684\u4f18\u52bf\uff0c\u5e7f\u5dde\u5149\u4eea\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8\u63a8\u51fa\u4e86LASER \u7cfb\u5217\u591a\u6a21\u5f0f\u5c0f\u52a8\u7269\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf\uff0c\u65e2\u80fd\u6355\u83b7\u6781\u5fae\u5f31\u7684\u751f\u7269\u53d1\u5149\u4fe1\u53f7\uff0c\u4e5f\u53ef\u5b9e\u73b0\u591a\u901a\u9053\u8367\u5149\u6210\u50cf\uff0c\u6ee1\u8db3\u80bf\u7624\u3001\u795e\u7ecf\u79d1\u5b66\u3001\u836f\u7269\u5206\u5e03\u7b49\u591a\u9886\u57df\u7814\u7a76\u9700\u6c42\u3002<\/span><\/p>\n\n\n\n
\n
    \n
  • \u591a\u6a21\u5f0f\u6210\u50cf<\/span><\/li>\n
  • \u9ad8\u7075\u654f\u6df1\u51b7\u80cc\u7167\u5f0f\u76f8\u673a<\/span><\/li>\n
  • \u9ad8\u6027\u80fd\u6ee4\u5149\u7247<\/span><\/li>\n
  • \u5927\u5149\u5708\u81ea\u52a8\u805a\u7126\u955c\u5934<\/span><\/li>\n
  • \u5927\u89c6\u91ce\u9ad8\u901a\u91cf<\/span><\/li>\n
  • \u5149\u8c31\u5206\u79bb<\/span><\/li>\n
  • \u591a\u5149\u6e90\u7cfb\u7edf<\/span><\/li>\n
  • \u5347\u964d\u5e73\u53f0\u4e0e\u9ebb\u9189\u7cfb\u7edf<\/span><\/li>\n
  • \u81ea\u52a8\u5316\u8f6f\u4ef6<\/span><\/li>\n<\/ul>\n<\/td>\n
\"\"<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

 <\/p>\n

\n
\n
\n
\u90e8\u5206\u53c2\u8003\u6587\u732e\uff1a<\/span>[1]Ow DW, Wood KV, DeLuca M, et al. Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science. 1986;234(4778):856-859. doi:10.1126\/science.234.4778.856<\/span>[2]Contag CH, Spilman SD, Contag PR, et al. Visualizing gene expression in living mammals using a bioluminescent reporter. Photochemistry and Photobiology. 1997;66(4):523-531. doi:10.1111\/j.1751-1097.1997.tb03184.x<\/span>[3]Heim R, Cubitt AB, Tsien RY. Improved green fluorescence. Nature. 1995;373:663\u2013664. doi:10.1038\/373663b0<\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n","protected":false},"excerpt":{"rendered":"

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McElroy\u56e2\u961f\u63ed\u793a\u4e86\u8424\u706b\u866b\u53d1\u5149\u7684\u5316\u5b66\u673a\u5236\uff0c\u786e\u5b9a\u8fd9\u662f\u4e00\u7c7b\u4f9d\u8d56 ATP \u7684\u9176\u4fc3\u53cd\u5e94\uff0c\u5e76\u6210\u529f\u5206\u79bb\u51fa\u5173\u952e\u53d1\u5149\u9176\uff0c\u81f3\u6b64\u751f\u7269\u81ea\u53d1\u5149\u7684\u673a\u5236\u57fa\u672c\u660e\u786e\u3002\u5176\u6838\u5fc3\u8fc7\u7a0b\u662f\uff1a\u8367\u5149\u7d20\u9176\u50ac\u5316\u8367\u5149\u7d20\u5728 ATP \u548c\u6c27\u6c14\u4f5c\u7528\u4e0b\u88ab\u6c27\u5316\u751f\u6210\u6fc0\u53d1\u6001\u7684\u4ea7\u7269\uff0c\u968f\u540e\u8be5\u4ea7\u7269\u56de\u5230\u57fa\u6001\u65f6\u91ca\u653e\u5149\u5b50\uff0c\u5b9e\u73b0\u4ece\u5316\u5b66\u80fd\u5230\u5149\u80fd\u7684\u8f6c\u5316\u3002 1980\u5e74\u4ee3\uff0c\u5206\u5b50\u751f\u7269\u5b66\u5c06\u8be5\u4f53\u7cfb\u7528\u4e8e\u57fa\u56e0\u7814\u7a76\uff1aOw \u5c06\u8367\u5149\u7d20\u9176\u57fa\u56e0\u5bfc\u5165\u690d\u7269\uff0cde\u202fWet 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Contag\u56e2\u961f\u5229\u7528\u7ec6\u83cc\u53d1\u5149\u57fa\u56e0\u5728\u5c0f\u9f20\u4f53\u5185\u5b9e\u73b0\u975e\u4fb5\u5165\u6210\u50cf\uff1b1997\u5e74\uff0c\u4ed6\u4eec\u8fdb\u4e00\u6b65\u5c06\u8424\u706b\u866b\u4f53\u7cfb\u7528\u4e8e\u54fa\u4e73\u52a8\u7269\uff0c\u5b9e\u73b0\u4e86\u6d3b\u4f53\u57fa\u56e0\u8868\u8fbe\u53ef\u89c6\u5316\u3002\u8fd9\u4e00\u7a81\u7834\u4f7f\u7814\u7a76\u8005\u80fd\u591f\u52a8\u6001\u89c2\u5bdf\u80bf\u7624\u751f\u957f\u3001\u57fa\u56e0\u8868\u8fbe\u53ca\u836f\u7269\u4f5c\u7528\uff0c\u5927\u5e45\u62d3\u5c55\u4e86\u751f\u7269\u533b\u5b66\u7814\u7a76\u624b\u6bb5\u3002 \u56fe1.\u8424\u706b\u866b\u751f\u7269\u53d1\u5149\uff08\u6765\u6e90\u65b0\u6d6ahttps:\/\/k.sina.com.cn\/article_3857243723_pe5e8de4b0010057t7.html\uff09 \u56fe2.\u8424\u706b\u866b\u8367\u5149\u7d20\u9176\u5316\u5b66\u673a\u7406\u793a\u610f\uff08\u6765\u6e90\u77e5\u4e4ehttps:\/\/zhuanlan.zhihu.com\/p\/670401009\uff09 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